PMID- 1918959
OWN - NLM
STAT- MEDLINE
DCOM- 19911028
LR  - 20151119
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 147
IP  - 7
DP  - 1991 Oct 1
TI  - Production and characterization of mouse monoclonal antibodies against human 
      monocyte chemoattractant protein-1.
PG  - 2229-33
AB  - We developed five different hybridoma cell lines that produced mAb against human 
      monocyte chemoattractant protein-1 (MCP-1). The subclass of all five antibodies 
      was IgG1. All five mAb formed complexes with metabolically labeled MCP-1 that 
      could be demonstrated by immunoprecipitation. The antibodies were specific for 
      MCP-1. They did not cross-react by immunoprecipitation with structurally related 
      host defense cytokines present in metabolically labeled PHA- or LPS-stimulated 
      mononuclear cell culture fluids, nor did they cross-react in a direct ELISA with 
      neutrophil attractant/activation protein-1, with crude platelet lysate proteins, 
      or with pure platelet proteins that have amino acids sequences similar to that of 
      MCP-1. The mAb also reacted with rMCP-1 expressed in Escherichia coli, suggesting 
      that they recognize protein structure rather than the glycosylated portion of 
      human MCP-1. When the mAb were mixed with MCP-1, the monocyte chemotactic 
      response to MCP-1 was inhibited. A sandwich ELISA was developed to detect MCP-1 
      in biologic fluids containing relatively high concentrations of other proteins. 
      The sensitivity was 300 pg/ml, or 30 pg/ELISA well. An anti-MCP-1 mAb column was 
      used in an improved method of MCP-1 purification. Approximately 240 micrograms of 
      MCP-1 were purified from 5 liters of FCS-containing U-105MG cell culture 
      supernatant. The yield was at least 60%. In addition to two forms of MCP-1 
      reported previously by us, two more forms of MCP-1 were found in a mixture of 
      culture supernatants of PHA- and LPS-stimulated human PBMC.
FAU - Yoshimura, T
AU  - Yoshimura T
AD  - Immunopathology Section, National Cancer Institute-Frederick Cancer Research and 
      Development Center, MD 21702.
FAU - Takeya, M
AU  - Takeya M
FAU - Takahashi, K
AU  - Takahashi K
FAU - Kuratsu, J
AU  - Kuratsu J
FAU - Leonard, E J
AU  - Leonard EJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemotactic Factors)
RN  - 0 (Immunoglobulin G)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/immunology/*isolation & purification
MH  - Chemokine CCL2
MH  - Chemotactic Factors/analysis/*immunology/isolation & purification
MH  - Chemotaxis, Leukocyte
MH  - Chromatography, Affinity
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Immunoglobulin G/immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Monocytes/immunology
MH  - Precipitin Tests
EDAT- 1991/10/01 00:00
MHDA- 1991/10/01 00:01
CRDT- 1991/10/01 00:00
PHST- 1991/10/01 00:00 [pubmed]
PHST- 1991/10/01 00:01 [medline]
PHST- 1991/10/01 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1991 Oct 1;147(7):2229-33.