PMID- 19190682
OWN - NLM
STAT- MEDLINE
DCOM- 20090626
LR  - 20240326
IS  - 1673-7067 (Print)
IS  - 1995-8218 (Electronic)
IS  - 1995-8218 (Linking)
VI  - 25
IP  - 1
DP  - 2009 Feb
TI  - Central administration of Orphanin FQ inhibits GnRH secretion by ORL1 receptor in 
      the median eminence of freely moving ovariectomized rats.
PG  - 1-6
LID - 10.1007/s12264-009-1119-5 [doi]
LID - 1
AB  - OBJECTIVE: This study aimed to investigate the possible role of Orphanin FQ (OFQ) 
      in the regulation of hypo-thalamic gonadotropin-releasing hormone (GnRH) 
      secretion. METHODS: The method of push-pull perfusion and radioimmuno-assay (RIA) 
      were adopted to examine the secretory profile of GnRH in the median eminence (ME) 
      in freely moving ovari-ectomized (OVX) rats after intracerebroventricular (icv) 
      injection of OFQ and/or [Nphe(1)]NC(1-13)NH(2) (NC13), a competitive antagonists 
      of the opioid receptor-like 1 receptor (ORL1 receptor). RESULTS: GnRH release 
      from ME significantly decreased from 40 min to 80 min after the administration of 
      20 and 200 nmol OFQ in OVX rats (P < 0.05). This inhibitory effect of 20 nmol OFQ 
      could be abolished by pretreatment with equal dose of NC13. More interestingly, 
      GnRH secretion from ME was increased markedly 60 min after icv injection of 100 
      and 200 nmol NC13 (P < 0.05). CONCLUSION: Our results suggested central 
      administration of OFQ could inhibit the release of GnRH in the ME of hypothalamus 
      through ORL1 receptor, providing further in vivo evidence supporting the role of 
      OFQ in the control of GnRH secretion.
FAU - An, Xiao-Fei
AU  - An XF
AD  - Department of Endocrinology, Jiangsu Province Hospital of Traditional Chinese 
      Med, Nanjing 210029, China. anxiaofei2000@163.com
FAU - He, Ming
AU  - He M
FAU - Feng, Yi
AU  - Feng Y
FAU - Feng, Hao
AU  - Feng H
FAU - Yu, Jiang-Yi
AU  - Yu JY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Singapore
TA  - Neurosci Bull
JT  - Neuroscience bulletin
JID - 101256850
RN  - 0 (Narcotic Antagonists)
RN  - 0 (Opioid Peptides)
RN  - 0 (Peptide Fragments)
RN  - 0 (Receptors, Opioid)
RN  - 0 (Vasodilator Agents)
RN  - 0 (nociceptin-(1-13)-NH2, NPhe(1)-)
RN  - 33515-09-2 (Gonadotropin-Releasing Hormone)
RN  - 0 (Nociceptin Receptor)
RN  - 0 (Oprl protein, rat)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Gonadotropin-Releasing Hormone/*metabolism
MH  - Median Eminence/*metabolism
MH  - Narcotic Antagonists
MH  - Opioid Peptides/*pharmacology
MH  - Ovariectomy/methods
MH  - Peptide Fragments/pharmacology
MH  - Radioimmunoassay
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Opioid/*metabolism
MH  - Secretory Pathway/*drug effects
MH  - Vasodilator Agents/*pharmacology
MH  - Wakefulness/*physiology
MH  - Nociceptin Receptor
MH  - Nociceptin
PMC - PMC5552497
EDAT- 2009/02/05 09:00
MHDA- 2009/06/27 09:00
PMCR- 2010/02/01
CRDT- 2009/02/05 09:00
PHST- 2009/02/05 09:00 [entrez]
PHST- 2009/02/05 09:00 [pubmed]
PHST- 2009/06/27 09:00 [medline]
PHST- 2010/02/01 00:00 [pmc-release]
AID - 1119 [pii]
AID - 10.1007/s12264-009-1119-5 [doi]
PST - ppublish
SO  - Neurosci Bull. 2009 Feb;25(1):1-6. doi: 10.1007/s12264-009-1119-5.