PMID- 19190855
OWN - NLM
STAT- MEDLINE
DCOM- 20090414
LR  - 20090225
IS  - 1420-908X (Electronic)
IS  - 1023-3830 (Linking)
VI  - 58
IP  - 3
DP  - 2009 Mar
TI  - Telopeptides of type II collagen upregulate proteinases and damage cartilage but 
      are less effective than highly active fibronectin fragments.
PG  - 161-9
LID - 10.1007/s00011-009-8090-5 [doi]
AB  - OBJECTIVE AND DESIGN: We hypothesize that N-telopeptide (NT) and C-telopeptides 
      (CT) of type II collagen can enhance proteinases and cause cartilage damage and 
      have compared damaging activities to an extensively characterized potent 
      fibronectin fragment (Fn-f). MATERIALS: NT and CT peptides were synthesized. 
      METHODS: Interaction of labeled peptides with chondrocytes was studied by 
      fluorescence microscopy. Effects on the metalloproteinases (MMPs) MMP-3 and 
      MMP-13 and on ADAMTS-5 were analyzed by western blotting. Cartilage damage was 
      assayed by loss of proteoglycan (PG) from cultured explants. RESULTS: NT and CT 
      peptides penetrated cartilage, bound to chondrocytes and enhanced proteinase 
      release and cartilage PG depletion. Peptides had detectable activity at 0.3 
      microM (1 microg/ml) and were comparable at 30 microM (100 microg/ml) to 1 microM 
      Fn-f (29 microg/ml). However, while the Fn-f enhanced IL-1beta and TNF-alpha, the 
      NT and CT peptides did not. CONCLUSIONS: Collagen peptides containing NT and CT 
      regions were less active on a molar basis than Fn-fs but were still potent 
      damaging agents. Since collagen fragments are found in OA cartilage at microg/ml, 
      they have the potential to play a role in physiologic cartilage damage.
FAU - Guo, D
AU  - Guo D
AD  - Department of Biochemistry and Molecular Biology, University of North Dakota 
      School of Medicine and Health Sciences, Grand Forks, 58202, USA.
FAU - Ding, L
AU  - Ding L
FAU - Homandberg, G A
AU  - Homandberg GA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Switzerland
TA  - Inflamm Res
JT  - Inflammation research : official journal of the European Histamine Research 
      Society ... [et al.]
JID - 9508160
RN  - 0 (Collagen Type II)
RN  - 0 (Fibronectins)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Peptide Fragments)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 3.4.24.- (ADAM Proteins)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
SB  - IM
MH  - ADAM Proteins/metabolism
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cartilage, Articular/metabolism/*pathology
MH  - Cattle
MH  - Cells, Cultured
MH  - Chondrocytes/cytology/metabolism
MH  - Collagen Type II/genetics/*metabolism
MH  - Fibronectins/genetics/*metabolism
MH  - Humans
MH  - Interleukin-1beta/immunology
MH  - Matrix Metalloproteinases/metabolism
MH  - Molecular Sequence Data
MH  - Osteoarthritis/metabolism/pathology
MH  - Peptide Fragments/genetics/*metabolism
MH  - Tumor Necrosis Factor-alpha/immunology
EDAT- 2009/02/05 09:00
MHDA- 2009/04/15 09:00
CRDT- 2009/02/05 09:00
PHST- 2009/02/05 09:00 [entrez]
PHST- 2009/02/05 09:00 [pubmed]
PHST- 2009/04/15 09:00 [medline]
AID - 10.1007/s00011-009-8090-5 [doi]
PST - ppublish
SO  - Inflamm Res. 2009 Mar;58(3):161-9. doi: 10.1007/s00011-009-8090-5.