PMID- 19191138
OWN - NLM
STAT- MEDLINE
DCOM- 20090220
LR  - 20220408
IS  - 1532-4303 (Electronic)
IS  - 0277-0903 (Linking)
VI  - 46
IP  - 1
DP  - 2009 Feb
TI  - Are genes associated with energy metabolism important in asthma and BMI?
PG  - 53-8
LID - 10.1080/02770900802460514 [doi]
AB  - OBJECTIVE: Increased serum leptin levels have been observed in asthmatic 
      patients. Leptin, via proliferation and activation of Th2 cells, may induce 
      inflammation in asthma. It has also been demonstrated that leptin mRNA expression 
      and protein levels increase in response to inflammatory stimuli. We hypothesized 
      that polymorphisms in the leptin receptor, leptin and ghrelin genes, may affect 
      their expression and, therefore, be responsible for altered response to increased 
      leptin levels observed in asthma. To our knowledge, there were no studies on a 
      potential role of LEPR, LEP, and GHRL polymorphisms in asthma. SUBJECTS AND 
      METHODS: We analyzed 129 pediatric patients with asthma and 114 healthy children 
      from the control group ranging from 6 to 18 years of age. The diagnosis of 
      allergic asthma was based on clinical symptoms, the lung function test, and the 
      positive skin prick test and/or increased immunoglobulin E (IgE) levels. 
      Polymorphisms were genotyped by the polymerase chain reaction-restriction 
      fragment length polymorphism (PCR-RFLP) method. Statistical analyses were 
      performed with Statistica v.7.1 software (Statistica, StatSoft, Poland; available 
      free at http://www.broad.mit.edu/mpg/haploview/index.php). Linkage disequilibrium 
      analysis was performed with Haploview v.4.0. RESULTS: We observed a statistically 
      significant association of the 3'UTR A/G and the -2549A/G polymorphisms of the 
      leptin gene with asthma. No association with asthma was observed for the K109R 
      and the Q223R polymorphisms of the LEPR gene and the Met72Leu polymorphism of the 
      ghrelin gene. In the analysis of body mass index (BMI) stratified by genotype, we 
      found an association of the -2549A/G LEP, but not of LEPR and GHRL polymorphisms, 
      with higher BMI values in asthmatic patients. We found suggestive evidence for 
      linkage between the two polymorphisms of the LEPR gene (D' = 0.84 CI: 0.71-0.92; 
      r(2) = 0.29) in linkage disequilibrium analysis: The GG haplotype was more 
      frequent in the control healthy group (p = 0.057). No linkage disequilibrium was 
      detected between LEP polymorphisms. CONCLUSIONS: Polymorphisms of the leptin gene 
      may be associated with asthma and higher BMI in asthmatic patients. Polymorphisms 
      of the LEPR and GHRL seem unlikely to be associated with asthma or BMI in our 
      sample. The results of haplotype analysis for the LEPR gene may suggest a 
      protective role of the GG haplotype against asthma; however, studies on larger 
      groups are necessary to confirm the observed trend towards association.
FAU - Szczepankiewicz, Aleksandra
AU  - Szczepankiewicz A
AD  - Clinics of Pediatric Pulmonology, Allergy and Clinical Immunology, Poznan 
      University of Medical Sciences, Poznan, Poland. alszczep@amp.edu.pl
FAU - Breborowicz, Anna
AU  - Breborowicz A
FAU - Sobkowiak, Paulina
AU  - Sobkowiak P
FAU - Popiel, Anna
AU  - Popiel A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Asthma
JT  - The Journal of asthma : official journal of the Association for the Care of 
      Asthma
JID - 8106454
RN  - 0 (Ghrelin)
RN  - 0 (Leptin)
RN  - 0 (Receptors, Leptin)
SB  - IM
MH  - Adolescent
MH  - Asthma/*genetics/metabolism
MH  - *Body Mass Index
MH  - Child
MH  - Energy Metabolism
MH  - Female
MH  - Gene Frequency
MH  - Genotype
MH  - Ghrelin/*genetics
MH  - Haplotypes
MH  - Humans
MH  - Leptin/*genetics
MH  - Linkage Disequilibrium
MH  - Male
MH  - Poland
MH  - Polymorphism, Single Nucleotide
MH  - Receptors, Leptin/*genetics
EDAT- 2009/02/05 09:00
MHDA- 2009/02/21 09:00
CRDT- 2009/02/05 09:00
PHST- 2009/02/05 09:00 [entrez]
PHST- 2009/02/05 09:00 [pubmed]
PHST- 2009/02/21 09:00 [medline]
AID - 908458384 [pii]
AID - 10.1080/02770900802460514 [doi]
PST - ppublish
SO  - J Asthma. 2009 Feb;46(1):53-8. doi: 10.1080/02770900802460514.