PMID- 1919185
OWN - NLM
STAT- MEDLINE
DCOM- 19911106
LR  - 20110729
IS  - 0300-9165 (Print)
IS  - 0300-9165 (Linking)
VI  - 43
IP  - 9
DP  - 1991 Sep
TI  - [Fertilizing capacity of fresh and frozen spermatozoa].
PG  - 1233-40
AB  - Although frozen semen is now being used clinically, the fertilizing function of 
      thawed semen has not yet been evaluated adequately. The changes in frozen sperm 
      functions were evaluated and the changes in regard to the fertilization 
      phenomenon were also investigated. The collected semen was evaluated by the 
      following function tests, 1) Bovine cervical Mucus Penetration test (BMP test), 
      2) Zona-free hamster egg Sperm Penetration Test (ZSPT), 3) Hypoosmotic Swelling 
      test (HOS test), 4) Triple stain technique (AR test), 5) Semen Auto Analyzer. The 
      freezing medium was KS-II solution with a program freezer (CRYO-10). The results 
      were as follows: 1) In the BMP test, the frozen normospermic group maintained 
      fertilizing capacity. 2) There was no significant difference between fresh and 
      frozen semen in the penetration rate of ZSPT except in the oligospermic group. 3) 
      In the HOS test, there was no difference between fresh and frozen specimens in 
      the number of swollen sperms which endured freezing and maintained the sperm 
      membrane integrity. 4) There was a tendency to compare to that of fresh semen the 
      increase in AR in frozen semen, but it was not significant. 5) There were low 
      values after thawing except for LHD, but there still remained fertilizability 
      after freezing. In conclusion, there was no reduction in fertilizing capacity 
      following freezing through out the sperm function tests.
FAU - Yoshida, H
AU  - Yoshida H
AD  - Department of Obstetrics and Gynecology, Tohoku University School of Medicine, 
      Sendai.
LA  - jpn
PT  - Comparative Study
PT  - English Abstract
PT  - Journal Article
PL  - Japan
TA  - Nihon Sanka Fujinka Gakkai Zasshi
JT  - Nihon Sanka Fujinka Gakkai zasshi
JID - 7505749
SB  - IM
MH  - Cryopreservation
MH  - Fertilization
MH  - Humans
MH  - Male
MH  - Spermatozoa/*physiology
EDAT- 1991/09/01 00:00
MHDA- 1991/09/01 00:01
CRDT- 1991/09/01 00:00
PHST- 1991/09/01 00:00 [pubmed]
PHST- 1991/09/01 00:01 [medline]
PHST- 1991/09/01 00:00 [entrez]
PST - ppublish
SO  - Nihon Sanka Fujinka Gakkai Zasshi. 1991 Sep;43(9):1233-40.