PMID- 19191955
OWN - NLM
STAT- MEDLINE
DCOM- 20100416
LR  - 20090323
IS  - 1365-2672 (Electronic)
IS  - 1364-5072 (Linking)
VI  - 106
IP  - 3
DP  - 2009 Mar
TI  - Bacterial and archaeal assemblages in sediments of a large shallow freshwater 
      lake, Lake Taihu, as revealed by denaturing gradient gel electrophoresis.
PG  - 1022-32
LID - 10.1111/j.1365-2672.2008.04069.x [doi]
AB  - AIMS: To explore the association of microbial community structure with the 
      development of eutrophication in a large shallow freshwater lake, Lake Taihu. 
      METHODS AND RESULTS: The bacterial and archaeal assemblages in sediments of 
      different lake areas were analysed using denaturing gradient gel electrophoresis 
      (DGGE) of amplified 16S rDNA fragments. The bacterial DGGE profiles showed that 
      eutrophied sites, grass-bottom areas and relatively clean sites with a eutrophic 
      (albeit dredged) site are three respective clusters. Fifty-one dominant bacterial 
      DGGE bands were detected and 92 corresponding clones were sequenced, most of 
      which were affiliated with bacterial phylotypes commonly found in freshwater 
      ecosystems. Actinobacteria were detected in the centre of the lake and not at 
      eutrophied sites whereas the opposite was found with respect to 
      Verrucomicrobiales. Twenty-five dominant archaeal DGGE bands were detected and 31 
      corresponding clones were sequenced, most of which were affiliated with 
      freshwater archaeal phylotypes. CONCLUSIONS: The bacterial community structures 
      in the sediments of different areas with similar water quality and situation tend 
      to be similar in Taihu Lake. SIGNIFICANCE AND IMPACT OF THE STUDY: This study may 
      expand our knowledge on the relationship between the overall microbial 
      assemblages and the development of eutrophication in the shallow freshwater lake.
FAU - Liu, F H
AU  - Liu FH
AD  - Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, 
      Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 
      Shanghai, China.
FAU - Lin, G H
AU  - Lin GH
FAU - Gao, G
AU  - Gao G
FAU - Qin, B Q
AU  - Qin BQ
FAU - Zhang, J S
AU  - Zhang JS
FAU - Zhao, G P
AU  - Zhao GP
FAU - Zhou, Z H
AU  - Zhou ZH
FAU - Shen, J H
AU  - Shen JH
LA  - eng
PT  - Journal Article
DEP - 20090121
PL  - England
TA  - J Appl Microbiol
JT  - Journal of applied microbiology
JID - 9706280
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Archaeal)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Archaea/classification/genetics/*isolation & purification
MH  - Bacteria/classification/genetics/*isolation & purification
MH  - DNA, Bacterial/analysis/genetics
MH  - DNA, Ribosomal/analysis/chemistry/genetics
MH  - Electrophoresis, Polyacrylamide Gel/methods
MH  - Eutrophication/physiology
MH  - *Fresh Water
MH  - Gene Library
MH  - Geologic Sediments/*microbiology
MH  - Molecular Sequence Data
MH  - RNA, Archaeal/genetics
MH  - RNA, Bacterial/genetics
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - *Water Microbiology
EDAT- 2009/02/05 09:00
MHDA- 2010/04/17 06:00
CRDT- 2009/02/05 09:00
PHST- 2009/02/05 09:00 [entrez]
PHST- 2009/02/05 09:00 [pubmed]
PHST- 2010/04/17 06:00 [medline]
AID - JAM4069 [pii]
AID - 10.1111/j.1365-2672.2008.04069.x [doi]
PST - ppublish
SO  - J Appl Microbiol. 2009 Mar;106(3):1022-32. doi: 10.1111/j.1365-2672.2008.04069.x. 
      Epub 2009 Jan 21.