PMID- 19191958
OWN - NLM
STAT- MEDLINE
DCOM- 20100416
LR  - 20090323
IS  - 1365-2672 (Electronic)
IS  - 1364-5072 (Linking)
VI  - 106
IP  - 3
DP  - 2009 Mar
TI  - Acrebol, a novel toxic peptaibol produced by an Acremonium exuviarum indoor 
      isolate.
PG  - 909-23
LID - 10.1111/j.1365-2672.2008.04062.x [doi]
AB  - AIMS: To identify a toxin and its producer isolated from woody material in a 
      building where the occupants experienced serious ill health symptoms. METHODS AND 
      RESULTS: Hyphal extracts of an indoor fungus, identified as the 
      cycloheximide-tolerant species Acremonium exuviarum, inhibited motility of boar 
      spermatozoa (EC(50) 5 +/- 2 microg of crude solids ml(-1)) and caused cytolysis 
      of murine neuroblastoma cells (MNA) and feline fetal lung cells (FL). The 
      responsible substances were purified and identified as two structurally similar, 
      heat-stable, novel, toxic peptaibols, 1726 Da and 1740 Da, respectively, with 
      amino acid sequences of 
      Acetyl-Phe-Iva/Val-Gln-Aib-Ile-Thr-Leu-Aib-Pro-Aib-Gln-Pro-Aib-(X-X-X)-SerOH and 
      Acetyl-Phe-Iva/Val-Gln-Aib-Ile-Thr-Leu-Val-Pro-Aib-Gln-Pro-Aib-(X-X-X)-SerOH. 
      Purified acrebol inhibited motility of boar sperm, depleted ATP half-content in 1 
      day (EC(50) of 0.1 microg ml(-1), 60 nmol l(-1)) depolarised the mitochondria 
      after 2 days, but did not affect the cellular content in NADH. This indicates 
      mitochondrial toxicity. Plate-grown biomass of A. exuviarum BMB4 contained 0.1-1% 
      (w/w) of acrebol, depending on the culture medium. CONCLUSIONS: Acrebol paralysed 
      the energy generation of mammalian cells suggesting that mitochondria were its 
      target of action. SIGNIFICANCE AND IMPACT OF THE STUDY: Acremonium exuviarum, as 
      an indoor fungus, is potentially hazardous to health because of the toxic 
      peptaibols that it produces.
FAU - Andersson, M A
AU  - Andersson MA
AD  - Department of Applied Chemistry and Microbiology, University of Helsinki, 
      Helsinki, Finland.
FAU - Mikkola, R
AU  - Mikkola R
FAU - Raulio, M
AU  - Raulio M
FAU - Kredics, L
AU  - Kredics L
FAU - Maijala, P
AU  - Maijala P
FAU - Salkinoja-Salonen, M S
AU  - Salkinoja-Salonen MS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090121
PL  - England
TA  - J Appl Microbiol
JT  - Journal of applied microbiology
JID - 9706280
RN  - 0 (Bacterial Toxins)
RN  - 0 (Peptaibols)
SB  - IM
MH  - Acremonium/*chemistry/metabolism
MH  - Amino Acid Sequence
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Bacterial Toxins/toxicity
MH  - Cats
MH  - Chromatography, High Pressure Liquid
MH  - Housing
MH  - Lung/drug effects/pathology
MH  - Male
MH  - Mass Spectrometry
MH  - Molecular Weight
MH  - Neuroblastoma/pathology
MH  - Peptaibols/*chemistry/pharmacology
MH  - Rats
MH  - Spermatozoa/drug effects
MH  - Wood/microbiology
EDAT- 2009/02/05 09:00
MHDA- 2010/04/17 06:00
CRDT- 2009/02/05 09:00
PHST- 2009/02/05 09:00 [entrez]
PHST- 2009/02/05 09:00 [pubmed]
PHST- 2010/04/17 06:00 [medline]
AID - JAM4062 [pii]
AID - 10.1111/j.1365-2672.2008.04062.x [doi]
PST - ppublish
SO  - J Appl Microbiol. 2009 Mar;106(3):909-23. doi: 10.1111/j.1365-2672.2008.04062.x. 
      Epub 2009 Jan 21.