PMID- 19192345
OWN - NLM
STAT- MEDLINE
DCOM- 20090501
LR  - 20190917
IS  - 1472-6491 (Electronic)
IS  - 1472-6483 (Linking)
VI  - 18
IP  - 2
DP  - 2009 Feb
TI  - Expression of certain HLA-I types in cleavage-stage embryos.
PG  - 244-50
AB  - This study examined the expression of human leukocyte antigen (HLA)-G and HLA-I 
      (which includes HLA-A, -B, -C, -E and -F, but is without HLA-G) in the cleavage 
      embryo and its supernatant, and related the results to embryo development 
      including growth rate and grade. In total, 136 day-3 cleavage embryos were used 
      for detection of HLA-G and 24 embryos for HLA-I without HLA-G by 
      immunohistochemistry. The expression of HLA-I was examined by western blot in the 
      lysates of a further 63 day-3 cleavage embryos; soluble HLA-I in the culture 
      supernatant of embryos with detectable HLA-I was also examined by western blot. 
      It was found that 90 of 136 (66.2%) cleavage embryos expressed HLA-G, whereas 23 
      of 24 (95.8%) embryos expressed HLA-I without HLA-G. HLA-G expression typically 
      showed an even and symmetrical pattern of distribution in each blastomere. HLA-I 
      without HLA-G in cleavage-stage embryos is typically scattered around the 
      blastomere surface. The expression of HLA-G but without HLA-I in cleavage-stage 
      embryos was significantly associated with embryo grade (P < 0.001) and cell 
      number (P = 0.03). In conclusion, HLA-I is expressed on day-3 cleavage embryos, 
      and HLA-G expression on preimplantation embryos is related to embryo development, 
      including embryo growth rate and embryo grade.
FAU - Wang, Qiong
AU  - Wang Q
AD  - Reproductive Medicine Centre, First Affiliated Hospital of Sun Yat-sen 
      University, Guangzhou 510080, China. wangqionggz@yahoo.com.cn
FAU - Zhuang, Guang-Lun
AU  - Zhuang GL
FAU - Zhou, Can-Quan
AU  - Zhou CQ
FAU - Li, Tin-Chiu
AU  - Li TC
FAU - Li, Jie
AU  - Li J
FAU - Xu, Yan-Wen
AU  - Xu YW
FAU - Gu, Xiong-Fei
AU  - Gu XF
FAU - Li, Yu-Bin
AU  - Li YB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Reprod Biomed Online
JT  - Reproductive biomedicine online
JID - 101122473
RN  - 0 (Culture Media)
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-G Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
SB  - IM
MH  - Blastocyst/cytology/metabolism
MH  - Cell Count
MH  - Cells, Cultured
MH  - Cleavage Stage, Ovum/*metabolism
MH  - Culture Media/chemistry/metabolism
MH  - Embryo, Mammalian/cytology/*metabolism
MH  - Embryonic Development/genetics
MH  - Gene Expression Regulation, Developmental
MH  - HLA Antigens/genetics/metabolism
MH  - HLA-G Antigens
MH  - Histocompatibility Antigens Class I/analysis/*genetics/metabolism
MH  - Humans
MH  - Quality Control
MH  - Time Factors
EDAT- 2009/02/05 09:00
MHDA- 2009/05/02 09:00
CRDT- 2009/02/05 09:00
PHST- 2009/02/05 09:00 [entrez]
PHST- 2009/02/05 09:00 [pubmed]
PHST- 2009/05/02 09:00 [medline]
AID - S1472-6483(10)60262-3 [pii]
AID - 10.1016/s1472-6483(10)60262-3 [doi]
PST - ppublish
SO  - Reprod Biomed Online. 2009 Feb;18(2):244-50. doi: 10.1016/s1472-6483(10)60262-3.