PMID- 1919362
OWN - NLM
STAT- MEDLINE
DCOM- 19911031
LR  - 20190516
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 50
IP  - 4
DP  - 1991 Oct
TI  - Autonomous growth of human T-lymphotropic virus type I infected human lymphocytes 
      treated with N-methyl-N'-nitro-N-nitrosoguanidine and ultraviolet rays.
PG  - 341-8
AB  - Human T-cell cultures infected with human T-lymphotropic virus type I (HTLV-I) 
      and interleukin-2 (IL-2)-dependent for their continuous growth were treated with 
      N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and then maintained in the medium 
      containing phorbol 12-myristate 13-acetate (TPA). Cells achieved independence 
      from IL-2 but became TPA-dependent for continuous growth. Multiple ultraviolet 
      (UV) irradiations of TPA-dependent cells resulted in their autonomous growth. 
      G-band karyotype analysis revealed multiple chromosomal abnormalities that were 
      seen in cells before and after MNNG treatment and UV irradiations, and those that 
      were only seen in autonomously growing cells. Viral expression was found to be 
      transiently enhanced in association with emergence of certain chromosomal 
      changes. Exposure of HTLV-I infected cells to certain mutagens may promote the 
      occurrence of the specific rearrangement of cellular genes responsible for 
      regulation of cellular and viral replication and may lead these cells to 
      neoplastic transformation.
FAU - Maruyama, K
AU  - Maruyama K
AD  - Department of Pathology, Chiba Cancer Center Research Institute, Japan.
FAU - Mochizuki, S
AU  - Mochizuki S
FAU - Kawamura, K
AU  - Kawamura K
FAU - Tamayama, C
AU  - Tamayama C
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (DNA, Viral)
RN  - 12H3O2UGSF (Methylnitronitrosoguanidine)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Cell Division/drug effects/radiation effects
MH  - Cell Transformation, Viral/drug effects/genetics/radiation effects
MH  - Cells, Cultured
MH  - Chromosomes/drug effects/radiation effects/ultrastructure
MH  - Combined Modality Therapy
MH  - DNA, Viral/drug effects/genetics/radiation effects
MH  - Female
MH  - HTLV-I Infections/*drug therapy/radiotherapy
MH  - Human T-lymphotropic virus 1/genetics/*growth & development/physiology
MH  - Humans
MH  - Karyotyping
MH  - Lymphocytes/drug effects/*microbiology/radiation effects
MH  - Male
MH  - Methylnitronitrosoguanidine/*pharmacology/therapeutic use
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - *Ultraviolet Rays
EDAT- 1991/10/01 00:00
MHDA- 1991/10/01 00:01
CRDT- 1991/10/01 00:00
PHST- 1991/10/01 00:00 [pubmed]
PHST- 1991/10/01 00:01 [medline]
PHST- 1991/10/01 00:00 [entrez]
AID - 10.1002/jlb.50.4.341 [doi]
PST - ppublish
SO  - J Leukoc Biol. 1991 Oct;50(4):341-8. doi: 10.1002/jlb.50.4.341.