PMID- 19193740
OWN - NLM
STAT- MEDLINE
DCOM- 20090824
LR  - 20090622
IS  - 1460-2385 (Electronic)
IS  - 0931-0509 (Linking)
VI  - 24
IP  - 7
DP  - 2009 Jul
TI  - Dendritic cells in renal biopsies of patients with ANCA-associated vasculitis.
PG  - 2151-6
LID - 10.1093/ndt/gfp019 [doi]
AB  - BACKGROUND: Dendritic cells (DCs) maintain immune tolerance and are able to 
      initiate immune responses. Their involvement in ANCA-associated vasculitis (AAV) 
      is unknown. In this study, the participation of DC subsets is investigated in 
      renal biopsies of AAV patients. METHOD: A total of 25 patients with biopsy-proven 
      AAV and five healthy controls (HC) with normal renal histology were included. 
      Renal biopsies were stained for mature (CD208), immature (CD209), plasmacytoid 
      (CD303) and Langerhans (CD1a) DC subsets. Furthermore, T-cells were stained using 
      a T-cell marker (CD3). The interstitial cellular infiltrate was graded 
      semi-quantitatively from 0+ (= absence of cells) to 3+ (= numerous cells). Within 
      the glomeruli, an absolute count was performed for positive cells. RESULTS: 
      CD208+ and CD209+ cells were found within patients' glomeruli but not in HC (1 
      +/- 0.3 versus 0.08 +/- 0.1 cells/glom; 2 +/- 0.3 versus 0.1 +/- 0.07 
      cells/glom). An average of 0.3 +/- 0.1 cell/glom expressed CD3 in patients while 
      few cells were found in HC (0.1 +/- 0.7 cell/glom). Focal interstitial cellular 
      infiltrates were observed in patients' biopsies but not in HC. Interstitial 
      infiltration with CD3+ and CD209+ cells was assessed at an average of 1+, but 
      some glomeruli and tubuli were surrounded by CD3+ and CD209+ cells forming 
      clusters. Serial sections revealed that CD209+ cells were present in CD3+ rich 
      areas. CONCLUSION: Both mature and immature glomerular DCs are found in renal 
      biopsies of patients with AAV. Immature DCs cluster with T-cells in interstitial 
      infiltrates in these biopsies. Since DCs form aggregates in T-cell areas, we 
      hypothesize that these cells interact with each other and are involved in 
      lymphoid neogenesis.
FAU - Wilde, Benjamin
AU  - Wilde B
AD  - Division of Clinical and Experimental Immunology, Department of Internal 
      Medicine, University Hospital Maastricht, Maastricht, The Netherlands.
FAU - van Paassen, Pieter
AU  - van Paassen P
FAU - Damoiseaux, Jan
AU  - Damoiseaux J
FAU - Heerings-Rewinkel, Petra
AU  - Heerings-Rewinkel P
FAU - van Rie, Henk
AU  - van Rie H
FAU - Witzke, Oliver
AU  - Witzke O
FAU - Tervaert, Jan Willem Cohen
AU  - Tervaert JW
LA  - eng
PT  - Journal Article
DEP - 20090204
PL  - England
TA  - Nephrol Dial Transplant
JT  - Nephrology, dialysis, transplantation : official publication of the European 
      Dialysis and Transplant Association - European Renal Association
JID - 8706402
RN  - 0 (Antibodies, Antineutrophil Cytoplasmic)
SB  - IM
MH  - Adult
MH  - Aged
MH  - *Antibodies, Antineutrophil Cytoplasmic
MH  - Biopsy
MH  - Dendritic Cells/*pathology
MH  - Female
MH  - Humans
MH  - Kidney Glomerulus/*pathology
MH  - Male
MH  - Middle Aged
MH  - Vasculitis/*immunology/*pathology
MH  - Young Adult
EDAT- 2009/02/06 09:00
MHDA- 2009/08/25 09:00
CRDT- 2009/02/06 09:00
PHST- 2009/02/06 09:00 [entrez]
PHST- 2009/02/06 09:00 [pubmed]
PHST- 2009/08/25 09:00 [medline]
AID - gfp019 [pii]
AID - 10.1093/ndt/gfp019 [doi]
PST - ppublish
SO  - Nephrol Dial Transplant. 2009 Jul;24(7):2151-6. doi: 10.1093/ndt/gfp019. Epub 
      2009 Feb 4.