PMID- 19195026
OWN - NLM
STAT- MEDLINE
DCOM- 20090818
LR  - 20090727
IS  - 1554-527X (Electronic)
IS  - 0736-0266 (Linking)
VI  - 27
IP  - 8
DP  - 2009 Aug
TI  - Insulin-like growth factor-I improves chondrogenesis of predifferentiated human 
      umbilical cord mesenchymal stromal cells.
PG  - 1109-15
LID - 10.1002/jor.20848 [doi]
AB  - Human umbilical cord mesenchymal stromal cells (hUCMSCs) are an attractive cell 
      source for tissue engineering with numerous advantages over other adult stem cell 
      sources, such as great expansion ability in vitro and extensive availability. The 
      objective of this 6-week study was to test the hypothesis that switching from 
      chondrogenic transforming growth factor-beta3 (TGF-beta3) to anabolic 
      insulin-like growth factor-I (IGF-I) at the 3-week time point would produce more 
      cartilage-like matrix than TGF-beta3 alone. hUCMSCs were seeded into polyglycolic 
      acid (PGA) scaffolds and then cultured in chondrogenic medium containing 
      TGF-beta3 for 3 weeks. The TGF-beta3-treated hUCMSCs were then exposed for 3 more 
      weeks to one of four different conditions: (1) continued in chondrogenic medium, 
      (2) control medium (no TGF-beta3), (3) control medium with 10 ng/ml IGF-I, or (4) 
      control medium with 100 ng/ml IGF-I. Compared to continuing with TGF-beta3, 
      switching to IGF-I increased collagen production, and furthermore increased both 
      collagen type II gene expression and immunostaining. In conclusion, the shift 
      from TGF-beta3 to IGF-I at week 3 resulted in a significant increase of 
      cartilage-like extracellular matrix, confirming our hypothesis.
CI  - Copyright 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.
FAU - Wang, Limin
AU  - Wang L
AD  - Department of Chemical and Petroleum Engineering, University of Kansas, 4132 
      Learned Hall, 1530 W. 15th Street, Lawrence, Kansas 66045, USA.
FAU - Detamore, Michael S
AU  - Detamore MS
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Orthop Res
JT  - Journal of orthopaedic research : official publication of the Orthopaedic 
      Research Society
JID - 8404726
RN  - 0 (Collagen Type I)
RN  - 0 (Collagen Type II)
RN  - 0 (Transforming Growth Factor beta3)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
SB  - IM
MH  - Cells, Cultured
MH  - Chondrogenesis/*drug effects
MH  - Collagen Type I/biosynthesis
MH  - Collagen Type II/biosynthesis
MH  - Female
MH  - Humans
MH  - Insulin-Like Growth Factor I/*pharmacology
MH  - Stromal Cells/*cytology/drug effects/metabolism
MH  - Transforming Growth Factor beta3/pharmacology
MH  - Umbilical Cord/cytology
EDAT- 2009/02/06 09:00
MHDA- 2009/08/19 09:00
CRDT- 2009/02/06 09:00
PHST- 2009/02/06 09:00 [entrez]
PHST- 2009/02/06 09:00 [pubmed]
PHST- 2009/08/19 09:00 [medline]
AID - 10.1002/jor.20848 [doi]
PST - ppublish
SO  - J Orthop Res. 2009 Aug;27(8):1109-15. doi: 10.1002/jor.20848.