PMID- 19195999
OWN - NLM
STAT- MEDLINE
DCOM- 20100817
LR  - 20191210
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 57
IP  - 5
DP  - 2009 Mar 11
TI  - Multiclass analysis of antibiotic residues in honey by ultraperformance liquid 
      chromatography-tandem mass spectrometry.
PG  - 1760-7
LID - 10.1021/jf8034572 [doi]
AB  - A method has been developed and validated for the simultaneous analysis of 
      different veterinary drug residues (macrolides, tetracyclines, quinolones, and 
      sulfonamides) in honey. Honey samples were dissolved with Na(2)EDTA, and 
      veterinary residues were extracted from the supernatant by solid-phase extraction 
      (SPE), using OASIS HLB cartridges. The separation and determination was carried 
      out by ultraperformance liquid chromatography coupled to tandem mass spectrometry 
      (UPLC-MS/MS), using an electrospay ionization source (ESI) in positive mode. Data 
      acquisition under MS/MS was achieved by applying multiple reaction monitoring 
      (MRM) of two ion transitions per compound to provide a high degree of sensitivity 
      and specificity. The method was validated, and mean recoveries were evaluated at 
      three concentration levels (10, 50, and 100 microg/kg), ranging from 70 to 120% 
      except for doxycycline, erythromycin, and tylmicosin with recovery higher than 
      50% at the three levels assayed. Relative standard deviations (RSDs) of the 
      recoveries were less than 20% within the intraday precision and less than 25% 
      within the interday precision. The limits of quantification (LOQs) were always 
      lower than 4 microg/kg. The developed procedure was applied to 16 honey samples, 
      and erythromycin, sarafloxacin, and tylosin were found in a few samples.
FAU - Vidal, Jose Luis Martinez
AU  - Vidal JL
AD  - Group "Analytical Chemistry of Contaminants", Department of Analytical Chemistry, 
      Almeria University, E-04071 Almeria, Spain.
FAU - Aguilera-Luiz, Maria Del Mar
AU  - Aguilera-Luiz Mdel M
FAU - Romero-Gonzalez, Roberto
AU  - Romero-Gonzalez R
FAU - Frenich, Antonia Garrido
AU  - Frenich AG
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Anti-Bacterial Agents)
SB  - IM
MH  - Anti-Bacterial Agents/*analysis
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Drug Residues/*analysis
MH  - Honey/*analysis
MH  - Tandem Mass Spectrometry/*methods
EDAT- 2009/02/07 09:00
MHDA- 2010/08/18 06:00
CRDT- 2009/02/07 09:00
PHST- 2009/02/07 09:00 [entrez]
PHST- 2009/02/07 09:00 [pubmed]
PHST- 2010/08/18 06:00 [medline]
AID - 10.1021/jf8034572 [pii]
AID - 10.1021/jf8034572 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2009 Mar 11;57(5):1760-7. doi: 10.1021/jf8034572.