PMID- 19196136
OWN - NLM
STAT- MEDLINE
DCOM- 20090916
LR  - 20151119
IS  - 1937-335X (Electronic)
IS  - 1937-3341 (Linking)
VI  - 15
IP  - 7
DP  - 2009 Jul
TI  - Practical induction system for dopamine-producing cells from bone marrow stromal 
      cells using spermine-pullulan-mediated reverse transfection method.
PG  - 1655-65
LID - 10.1089/ten.tea.2008.0453 [doi]
AB  - Introduction of various kinds of exogenous genes is an important step for control 
      of differentiation in stem cell biology and regenerative medicine. However, some 
      kinds of cells are vulnerable to manipulations such as gene delivery. In this 
      context, a gene introduction method with higher efficiency and safety is 
      required. Bone marrow stromal cells (BMSCs) offer possibilities for clinical 
      application because of their potential for expandability and ability to be 
      auto-transplanted. In this study, we established an efficient induction system of 
      dopamine-producing neuronal cells from BMSCs in several species using the 
      spermine-pullulan-mediated reverse transfection technique. In this system, 
      introduced exogenous plasmid genes were successfully transcribed and expressed as 
      proteins in the cytoplasm of BMSCs with the smallest number of cell death. 
      Microtubule-associated protein 2 and anti-beta-tubulin class III+ neurons were 
      successfully delivered from human, monkey, and mouse BMSCs, and further treatment 
      with trophic factors promoted differentiation of induced neuronal cells into 
      dopamine-producing cells that were positive for tyrosine hydroxylase and secreted 
      dopamine after high K+ stimulation in high-performance liquid chromatography 
      analysis. Our study indicates the availability of the reverse transfection method 
      for the induction of dopamine-producing neuronal cells from BMSCs, which is 
      expected to apply to cell-based therapy in Parkinson's disease.
FAU - Nagane, Kentaro
AU  - Nagane K
AD  - Department of Biomaterials, Field of Tissue Engineering, Institute for Frontier 
      Medical Sciences, Kyoto University, Kyoto, Japan.
FAU - Kitada, Masaaki
AU  - Kitada M
FAU - Wakao, Shohei
AU  - Wakao S
FAU - Dezawa, Mari
AU  - Dezawa M
FAU - Tabata, Yasuhiko
AU  - Tabata Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Tissue Eng Part A
JT  - Tissue engineering. Part A
JID - 101466659
RN  - 0 (Glucans)
RN  - 2FZ7Y3VOQX (Spermine)
RN  - 8ZQ0AYU1TT (pullulan)
RN  - 9007-49-2 (DNA)
RN  - EC 1.13.12.- (Luciferases)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*cytology/drug effects
MH  - Cell Shape/drug effects
MH  - Cell Survival/drug effects
MH  - DNA/genetics
MH  - Dopamine/*biosynthesis
MH  - Glucans/*pharmacology/toxicity
MH  - Humans
MH  - Intracellular Space/drug effects/metabolism
MH  - Luciferases
MH  - Macaca
MH  - Mice
MH  - Microscopy, Confocal
MH  - Neurons/cytology/drug effects/*metabolism
MH  - Plasmids/genetics
MH  - Species Specificity
MH  - Spermine/*pharmacology/toxicity
MH  - Stromal Cells/*cytology/drug effects/metabolism
MH  - Transfection/*methods
MH  - Transgenes
EDAT- 2009/02/07 09:00
MHDA- 2009/09/17 06:00
CRDT- 2009/02/07 09:00
PHST- 2009/02/07 09:00 [entrez]
PHST- 2009/02/07 09:00 [pubmed]
PHST- 2009/09/17 06:00 [medline]
AID - 10.1089/ten.tea.2008.0453 [pii]
AID - 10.1089/ten.tea.2008.0453 [doi]
PST - ppublish
SO  - Tissue Eng Part A. 2009 Jul;15(7):1655-65. doi: 10.1089/ten.tea.2008.0453.