PMID- 19196181
OWN - NLM
STAT- MEDLINE
DCOM- 20090925
LR  - 20181201
IS  - 1557-9042 (Electronic)
IS  - 0897-7151 (Linking)
VI  - 26
IP  - 1
DP  - 2009 Jan
TI  - Neural progenitor cells enhance the survival and axonal regeneration of injured 
      motoneurons after transplantation into the avulsed ventral horn of adult rats.
PG  - 67-80
LID - 10.1089/neu.2008.0656 [doi]
AB  - In the present study, we transplanted E13.5 spinal cord-derived neural progenitor 
      cells (NPCs) into the acutely avulsed ventral horn of adult rats. The results 
      showed that NPCs survived and integrated nicely within the host ventral horn at 6 
      weeks post-grafting. Although the majority of grafted NPCs differentiated into 
      astrocytes and only a small proportion into neuronal cells, interestingly, 
      grafted NPCs in the avulsed ventral horn significantly enhanced the survival of 
      injured motoneurons and promoted their regeneration into a peripheral nerve (PN) 
      graft, as revealed by retrograde FluoroGold (FG) labeling. Specific ELISAs, 
      Western blotting, and quantitative real-time reverse transcriptase polymerase 
      chain reaction (RT-PCR) demonstrated that NPCs produced nerve growth factor 
      (NGF), brain-derived neurotrophic factor (BDNF), and glial cell line-derived 
      neutrophilic factor (GDNF), both in vitro and after transplantation in vivo. 
      These results indicate that NPCs have beneficial effects on the survival and 
      axonal regeneration of avulsion-injured motoneurons after transplantation. Such 
      beneficial effects are possibly due to their inherent ability to secrete various 
      trophic factors after transplantation in vivo.
FAU - Su, Huanxing
AU  - Su H
AD  - Department of Anatomy, Li Ka Shing Faculty of Medicine, The University of Hong 
      Kong , Pokfulam, Hong Kong SAR, China.
FAU - Zhang, Wenming
AU  - Zhang W
FAU - Guo, Jiasong
AU  - Guo J
FAU - Guo, Anchen
AU  - Guo A
FAU - Yuan, Qiuju
AU  - Yuan Q
FAU - Wu, Wutian
AU  - Wu W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurotrauma
JT  - Journal of neurotrauma
JID - 8811626
RN  - 0 (Nerve Growth Factors)
SB  - IM
MH  - Animals
MH  - Cell Survival/physiology
MH  - Disease Models, Animal
MH  - Female
MH  - Graft Survival/physiology
MH  - Motor Neuron Disease/physiopathology/*therapy
MH  - Motor Neurons/*physiology
MH  - Nerve Growth Factors/genetics/metabolism
MH  - Nerve Regeneration/*physiology
MH  - Peripheral Nerves/cytology/physiology/transplantation
MH  - Radiculopathy/physiopathology/*therapy
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Rats, Transgenic
MH  - Recovery of Function/physiology
MH  - Spinal Cord/pathology/physiopathology/surgery
MH  - Stem Cell Transplantation/*methods
MH  - Stem Cells/cytology/*physiology
MH  - Tissue Transplantation/methods
MH  - Treatment Outcome
EDAT- 2009/02/07 09:00
MHDA- 2009/09/26 06:00
CRDT- 2009/02/07 09:00
PHST- 2009/02/07 09:00 [entrez]
PHST- 2009/02/07 09:00 [pubmed]
PHST- 2009/09/26 06:00 [medline]
AID - 10.1089/neu.2008.0656 [pii]
AID - 10.1089/neu.2008.0656 [doi]
PST - ppublish
SO  - J Neurotrauma. 2009 Jan;26(1):67-80. doi: 10.1089/neu.2008.0656.