PMID- 19197121
OWN - NLM
STAT- MEDLINE
DCOM- 20110404
LR  - 20090206
IS  - 1672-7347 (Print)
IS  - 1672-7347 (Linking)
VI  - 34
IP  - 1
DP  - 2009 Jan
TI  - [Identification of EBV chromosomal integration sites in Raji cells by 
      fluorescence in situ hybridization].
PG  - 13-9
AB  - OBJECTIVE: To identify the Epstein-Barr virus (EBV) chromosomal integration sites 
      in Raji cells. METHODS: EBV DNA was detected by Southern hybridization, and the 
      viral chromosomal integration sites were identified using G banding and 
      fluorescence in situ hybridization (FISH). RESULTS: BamHI-digested genomic DNA 
      from Raji cells was hybridized with (32)P-labeled probe-1 (EBV genome 13,232 
      approximately 16,189) and Probe-2 (EBV genome 5 approximately 3,271), which 
      generated 4 and 10, 23 kb positive bands respectively. The viral integration 
      sites included 1p, 1q, 2q, 3p, 3q, 4 q, 5q, 6q, 7p, 7q, 9q,11p, 14 q, and 15q,and 
      chromosomal bands 4 q, 2q, 1q and 7q were viral integration sites with high 
      frequencies. Among the 33 signals counted, 7, 4, 4,and 4 signals were at the site 
      4 q, 2q, 1q, and 7q respectively, and 64% of the total signals were found in 
      these 4 chromosomal bands. No viral integration occurred in chromosomes 16 
      approximately 22 or the sex chromosomes (X, Y). CONCLUSION: This study firstly 
      identifies the EBV integration sites in Raji cells using G banding and FISH. 
      There are some viral integration sites with high frequencies in Raji cells, and 
      EBV integrates into Raji cell genomes non-randomly.
FAU - Gao, Jianming
AU  - Gao J
AD  - Department of Pathophysiology, School of Medicine, Three Gorges University, 
      Yichang Hubei 443002,China.
FAU - Li, Xiaoling
AU  - Li X
FAU - Li, Guiyuan
AU  - Li G
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhong Nan Da Xue Xue Bao Yi Xue Ban
JT  - Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. 
      Medical sciences
JID - 101230586
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Burkitt Lymphoma/genetics/pathology/*virology
MH  - Cell Line, Tumor
MH  - Chromosomes, Human, Pair 1/virology
MH  - Chromosomes, Human, Pair 2/virology
MH  - Chromosomes, Human, Pair 4/virology
MH  - DNA, Viral/*genetics
MH  - *Genome, Viral
MH  - Herpesvirus 4, Human/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Virus Integration/*genetics
EDAT- 2009/02/07 09:00
MHDA- 2011/04/05 06:00
CRDT- 2009/02/07 09:00
PHST- 2009/02/07 09:00 [entrez]
PHST- 2009/02/07 09:00 [pubmed]
PHST- 2011/04/05 06:00 [medline]
AID - 1672-7347(2009)01-0013-07 [pii]
PST - ppublish
SO  - Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2009 Jan;34(1):13-9.