PMID- 19199536
OWN - NLM
STAT- MEDLINE
DCOM- 20090415
LR  - 20220129
IS  - 0022-1899 (Print)
IS  - 0022-1899 (Linking)
VI  - 199
IP  - 5
DP  - 2009 Mar 1
TI  - Association between tuberculin skin test reactivity, the memory CD4 cell subset, 
      and circulating FoxP3-expressing cells in HIV-infected persons.
PG  - 702-10
LID - 10.1086/596735 [doi]
AB  - BACKGROUND: Lack of reactivity to the tuberculin skin test (TST) is widely 
      observed in individuals with advanced human immunodeficiency virus type 1 (HIV-1) 
      infection. METHODS: Biopsy specimens from the TST reaction site and from skin not 
      infiltrated with purified protein derivative were obtained from 15 HIV-1-infected 
      and 23 uninfected persons who did not have active tuberculosis and who were from 
      a community in which the incidence of tuberculosis was very high. Histologic 
      sections (size, 8 mum) were immunohistochemically stained for CD4, CD8, CD28, 
      CD45RA, CD45RO, CD62L, CD1a, human leukocyte antigen (HLA)-DR, granulysin, 
      interferon-gamma, and FoxP3 and were analyzed by single-cell in situ digital 
      imaging. Peripheral blood mononuclear cells were analyzed using a 
      fluorescence-activated cell sorter. RESULTS: Biopsy specimens obtained from 
      TST-reactive skin of HIV-1-infected persons demonstrated fewer CD4(+) T cells at 
      the TST site (P = .36) but more HLA-DR(+) T cells (P = .037) than did such biopsy 
      specimens obtained from HIV-1-uninfected persons. Among HIV-1-infected persons, 
      the total number of cells (P = .008) and numbers of CD45RO(+) memory T cells (P = 
      .003) were significantly higher in TST-reactive persons than in TST-unreactive 
      persons. For HIV-1-infected persons, TST induration was inversely correlated with 
      the numbers of FoxP3(+) T cells in the blood (P = .026) but was unrelated to the 
      number of circulating CD4(+) T cells. CONCLUSIONS: For HIV-1 infected persons, 
      the TST depends on memory T cells and is more strongly associated with the 
      numbers of circulating FoxP3(+)CD4(+) T cells than with the total number of 
      CD4(+) T cells.
FAU - Sarrazin, Heike
AU  - Sarrazin H
AD  - Division of Clinical Infectious Diseases, Medical Clinic, Research Center 
      Borstel, Borstel, Germany.
FAU - Wilkinson, Katalin Andrea
AU  - Wilkinson KA
FAU - Andersson, Jan
AU  - Andersson J
FAU - Rangaka, Molebogeng Xheeda
AU  - Rangaka MX
FAU - Radler, Lena
AU  - Radler L
FAU - van Veen, Kerryn
AU  - van Veen K
FAU - Lange, Christoph
AU  - Lange C
FAU - Wilkinson, Robert John
AU  - Wilkinson RJ
LA  - eng
GR  - 072070/WT_/Wellcome Trust/United Kingdom
GR  - 084323/WT_/Wellcome Trust/United Kingdom
GR  - MC_U117588499/MRC_/Medical Research Council/United Kingdom
GR  - U117588499(88499)/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Infect Dis
JT  - The Journal of infectious diseases
JID - 0413675
RN  - 0 (FOXP3 protein, human)
RN  - 0 (Forkhead Transcription Factors)
SB  - IM
MH  - Adult
MH  - CD4-Positive T-Lymphocytes/classification/immunology/*physiology
MH  - Female
MH  - Forkhead Transcription Factors/genetics/*metabolism
MH  - HIV Infections/complications/*immunology
MH  - Humans
MH  - *Immunologic Memory
MH  - Immunophenotyping
MH  - Male
MH  - Skin/cytology/immunology
MH  - *Tuberculin Test
MH  - Tuberculosis/diagnosis
EDAT- 2009/02/10 09:00
MHDA- 2009/04/16 09:00
CRDT- 2009/02/10 09:00
PHST- 2009/02/10 09:00 [entrez]
PHST- 2009/02/10 09:00 [pubmed]
PHST- 2009/04/16 09:00 [medline]
AID - 10.1086/596735 [pii]
AID - 10.1086/596735 [doi]
PST - ppublish
SO  - J Infect Dis. 2009 Mar 1;199(5):702-10. doi: 10.1086/596735.