PMID- 19200187
OWN - NLM
STAT- MEDLINE
DCOM- 20090603
LR  - 20141120
IS  - 1365-4632 (Electronic)
IS  - 0011-9059 (Linking)
VI  - 48
IP  - 2
DP  - 2009 Feb
TI  - The anti-condyloma acuminatum effects of interferon-inducible protein 10 in 
      vitro.
PG  - 136-41
LID - 10.1111/j.1365-4632.2009.03776.x [doi]
AB  - BACKGROUND: Interferon-inducible protein 10 (IP-10) has been reported to show an 
      effective antiviral and antineoplastic role in various murine models. AIM: To 
      investigate the anti-condyloma acuminatum effect and human papillomavirus 
      (HPV)-inhibiting efficacy of a recombinant plasmid encoding IP-10 in vitro. 
      METHODS: A recombinant plasmid DNA carrying IP-10 cDNA was constructed. Condyloma 
      acuminatum tissue particles were transfected with IP-10 and examined for 
      apoptosis by terminal deoxynucleotidyl transferase-mediated dUTP nick end 
      labeling (TUNEL) staining and flow cytometry techniques. Relative real-time 
      polymerase chain reaction (PCR) was used to validate the HPV inhibited level of 
      the treatment groups. Immunohistochemistry was used to determine the IP-10 
      expression on condyloma acuminatum tissues. RESULTS: Transfected IP-10 was 
      expressed mainly in the cytoplasm of the condyloma acuminatum tissues. Plentiful 
      apoptosis was observed in condyloma acuminatum tissues transfected with IP-10. In 
      addition, HPV expression was lower in IP-10-treated tissues than in control 
      tissues. CONCLUSIONS: These observations suggest that IP-10 has strong 
      anti-condyloma acuminatum effects, inducing apoptosis and inhibiting HPV, and 
      therefore may be a novel and potentially effective therapy for condyloma 
      acuminatum.
FAU - Li, Huijuan
AU  - Li H
AD  - Department of Gynecology and Obstetrics, West China Second University Hospital, 
      and State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, 
      Sichuan University, Chengdu, Sichuan, China.
FAU - Yi, Tao
AU  - Yi T
FAU - Zhao, Shuyun
AU  - Zhao S
FAU - Chen, Pin
AU  - Chen P
FAU - Cheng, Chenxian
AU  - Cheng C
FAU - Wei, Yuquan
AU  - Wei Y
FAU - Zhao, Xia
AU  - Zhao X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Int J Dermatol
JT  - International journal of dermatology
JID - 0243704
RN  - 0 (CXCL10 protein, human)
RN  - 0 (Chemokine CXCL10)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Adult
MH  - Apoptosis
MH  - Chemokine CXCL10/*genetics
MH  - Condylomata Acuminata/*therapy/virology
MH  - Female
MH  - Flow Cytometry
MH  - Gene Expression
MH  - Genetic Therapy/*methods
MH  - *Human papillomavirus 11
MH  - *Human papillomavirus 6
MH  - Humans
MH  - In Situ Nick-End Labeling
MH  - In Vitro Techniques
MH  - Plasmids
MH  - Recombinant Proteins/genetics
MH  - Transfection
EDAT- 2009/02/10 09:00
MHDA- 2009/06/06 09:00
CRDT- 2009/02/10 09:00
PHST- 2009/02/10 09:00 [entrez]
PHST- 2009/02/10 09:00 [pubmed]
PHST- 2009/06/06 09:00 [medline]
AID - IJD3776 [pii]
AID - 10.1111/j.1365-4632.2009.03776.x [doi]
PST - ppublish
SO  - Int J Dermatol. 2009 Feb;48(2):136-41. doi: 10.1111/j.1365-4632.2009.03776.x.