PMID- 19200312
OWN - NLM
STAT- MEDLINE
DCOM- 20100326
LR  - 20090209
IS  - 1365-2672 (Electronic)
IS  - 1364-5072 (Linking)
VI  - 106
IP  - 2
DP  - 2009 Feb
TI  - Detection of heat-stable and heat-labile enterotoxin genes of Escherichia coli in 
      diarrhoeic faecal samples of mithun (Bos frontalis) calves by polymerase chain 
      reaction.
PG  - 455-8
LID - 10.1111/j.1365-2672.2008.04013.x [doi]
AB  - AIMS: To find out the prevalence of different serogroups of Escherichia coli (E. 
      coli) and to detect heat-stable (ST) and heat-labile (LT) enterotoxin genes of 
      enterotoxigenic E. coli (ETEC) from the faeces of mithun calves with diarrhoea. 
      METHODS AND RESULTS: Faecal samples obtained from 65 diarrhoeic mithun calves of 
      under 2 months of age were examined for E. coli using polymerase chain reaction 
      (PCR). Fifty-four E. coli isolates were obtained from those samples, which 
      belonged to 38 different serogroups. Out of 54 isolates tested by PCR, two 
      isolates (3.70%) belonging to serogroups O26 and O55 were found to possess gene 
      that code for ST enterotoxin and one isolate (1.85%) belonging to serogroup O125 
      was found to carry LT enterotoxin gene. CONCLUSIONS: Escherichia coli isolates 
      from diarrhoeic mithun calves were found to possess ST and LT enterotoxin genes, 
      which are designated as ETEC, and these isolates can be detected through PCR 
      using specific primers. SIGNIFICANCE AND IMPACT OF THE STUDY: This study reports 
      the isolation of ETEC possessing ST and LT enterotoxin genes for the first time 
      and ETEC could be a cause of diarrhoea in mithun calves leading to calf 
      mortality.
FAU - Rajkhowa, S
AU  - Rajkhowa S
AD  - Division of Animal Health, National Research Centre on Pig, Indian Council of 
      Agricultural Research, Rani, Guwahati, Assam, India. s_rajkhowa@lycos.com
FAU - Hussain, I
AU  - Hussain I
FAU - Rajkhowa, C
AU  - Rajkhowa C
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Appl Microbiol
JT  - Journal of applied microbiology
JID - 9706280
RN  - 0 (DNA, Bacterial)
RN  - 0 (Enterotoxins)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cattle Diseases/microbiology
MH  - DNA, Bacterial/genetics
MH  - Diarrhea/etiology/microbiology/veterinary
MH  - Enterotoxigenic Escherichia coli/classification/*genetics
MH  - Enterotoxins/analysis/*genetics
MH  - Escherichia coli Infections/microbiology/*veterinary
MH  - Feces/*microbiology
MH  - Genes, Bacterial
MH  - Polymerase Chain Reaction/methods/veterinary
MH  - Serotyping
EDAT- 2009/02/10 09:00
MHDA- 2010/03/27 06:00
CRDT- 2009/02/10 09:00
PHST- 2009/02/10 09:00 [entrez]
PHST- 2009/02/10 09:00 [pubmed]
PHST- 2010/03/27 06:00 [medline]
AID - JAM4013 [pii]
AID - 10.1111/j.1365-2672.2008.04013.x [doi]
PST - ppublish
SO  - J Appl Microbiol. 2009 Feb;106(2):455-8. doi: 10.1111/j.1365-2672.2008.04013.x.