PMID- 19200719 OWN - NLM STAT- MEDLINE DCOM- 20090526 LR - 20131121 IS - 1873-2976 (Electronic) IS - 0960-8524 (Linking) VI - 100 IP - 10 DP - 2009 May TI - Hydrogen production by immobilized R. faecalis RLD-53 using soluble metabolites from ethanol fermentation bacteria E. harbinense B49. PG - 2719-23 LID - 10.1016/j.biortech.2008.12.020 [doi] AB - In order to increase the hydrogen yield from glucose, hydrogen production by immobilized Rhodopseudomonas faecalis RLD-53 using soluble metabolites from ethanol fermentation bacteria Ethanoligenens harbinense B49 was investigated. The soluble metabolites from dark-fermentation mainly were ethanol and acetate, which could be further utilized for photo-hydrogen production. Hydrogen production by B49 was noticeably affected by the glucose and phosphate buffer concentration. The maximum hydrogen yield (1.83 mol H(2)/mol glucose) was obtained at 9 g/l glucose. In addition, we found that the ratio of acetate/ethanol (A/E) increased with increasing phosphate buffer concentration, which is favorable to further photo-hydrogen production. The total hydrogen yield during dark- and photo-fermentation reached its maximum value (6.32 mol H(2)/mol glucose) using 9 g/l glucose, 30 mmol/l phosphate buffers and immobilized R. faecalis RLD-53. Results demonstrated that the combination of dark- and photo- fermentation was an effective and efficient process to improve hydrogen yield from a single substrate. FAU - Liu, Bing-Feng AU - Liu BF AD - State Key Lab of Urban Water Resource and Environment, School of Municipal & Environmental Engineering, Harbin Institute of Technology, No. 202 Haihe Road, Harbin, China. FAU - Ren, Nan-Qi AU - Ren NQ FAU - Xing, De-Feng AU - Xing DF FAU - Ding, Jie AU - Ding J FAU - Zheng, Guo-Xiang AU - Zheng GX FAU - Guo, Wan-Qian AU - Guo WQ FAU - Xu, Ji-Fei AU - Xu JF FAU - Xie, Guo-Jun AU - Xie GJ LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20090205 PL - England TA - Bioresour Technol JT - Bioresource technology JID - 9889523 RN - 0 (Acetates) RN - 0 (Phosphates) RN - 3K9958V90M (Ethanol) RN - 7YNJ3PO35Z (Hydrogen) RN - IY9XDZ35W2 (Glucose) SB - IM MH - Acetates/*metabolism MH - Cells, Immobilized/metabolism MH - Clostridium/*metabolism MH - Ethanol/*metabolism MH - Fermentation MH - Glucose/*metabolism MH - Hydrogen/*metabolism MH - Phosphates/*metabolism MH - Rhodopseudomonas/*metabolism MH - Solubility EDAT- 2009/02/10 09:00 MHDA- 2009/05/27 09:00 CRDT- 2009/02/10 09:00 PHST- 2008/10/05 00:00 [received] PHST- 2008/12/10 00:00 [revised] PHST- 2008/12/10 00:00 [accepted] PHST- 2009/02/10 09:00 [entrez] PHST- 2009/02/10 09:00 [pubmed] PHST- 2009/05/27 09:00 [medline] AID - S0960-8524(08)01078-X [pii] AID - 10.1016/j.biortech.2008.12.020 [doi] PST - ppublish SO - Bioresour Technol. 2009 May;100(10):2719-23. doi: 10.1016/j.biortech.2008.12.020. Epub 2009 Feb 5.