PMID- 19200890
OWN - NLM
STAT- MEDLINE
DCOM- 20090402
LR  - 20240511
IS  - 1557-7988 (Electronic)
IS  - 0076-6879 (Print)
IS  - 0076-6879 (Linking)
VI  - 452
DP  - 2009
TI  - Methods to monitor chaperone-mediated autophagy.
PG  - 297-324
LID - 10.1016/S0076-6879(08)03619-7 [doi]
AB  - Chaperone-mediated autophagy (CMA) is a selective type of autophagy responsible 
      for the lysosomal degradation of soluble cytosolic proteins. In contrast to other 
      forms of autophagy where cargo is sequestered and delivered to lysosomes through 
      membrane fusion/excision, CMA substrates reach the lysosomal lumen after direct 
      translocation across the lysosomal membrane. CMA is part of the cellular quality 
      control systems and as such, essential for the cellular response to stress. CMA 
      activity decreases with age, likely contributing to the accumulation of altered 
      proteins characteristic in tissues from old organisms. Furthermore, impairment of 
      CMA underlies the pathogenesis of certain human pathologies such as 
      neurodegenerative disorders. These findings have drawn renewed attention to CMA 
      and a growing interest in the measurement of changes in CMA activity under 
      different physiological and pathological conditions. In this chapter we review 
      the different experimental approaches used to assess CMA activity both in cells 
      in culture and in different organs from animals.
FAU - Kaushik, Susmita
AU  - Kaushik S
AD  - Department of Developmental and Molecular Biology, Marion Bessin Liver Research 
      Center, Institute for Aging Research, Albert Einstein College of Medicine, Bronx, 
      New York, USA.
FAU - Cuervo, Ana Maria
AU  - Cuervo AM
LA  - eng
GR  - P30 DK041296/DK/NIDDK NIH HHS/United States
GR  - P01 DK041918/DK/NIDDK NIH HHS/United States
GR  - AG031782/AG/NIA NIH HHS/United States
GR  - R37 AG021904/AG/NIA NIH HHS/United States
GR  - R01 DK098408/DK/NIDDK NIH HHS/United States
GR  - R01 AG021904/AG/NIA NIH HHS/United States
GR  - DK041918/DK/NIDDK NIH HHS/United States
GR  - P01 AG031782/AG/NIA NIH HHS/United States
GR  - AG021904/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Enzymol
JT  - Methods in enzymology
JID - 0212271
RN  - 0 (HSC70 Heat-Shock Proteins)
RN  - 0 (Lysosomal-Associated Membrane Protein 2)
SB  - IM
MH  - Animals
MH  - Autophagy/*physiology
MH  - HSC70 Heat-Shock Proteins/metabolism
MH  - Immunoblotting
MH  - Lysosomal-Associated Membrane Protein 2/metabolism
MH  - Lysosomes/metabolism
MH  - Mice
MH  - Mice, Transgenic
PMC - PMC4300957
MID - NIHMS653873
EDAT- 2009/02/10 09:00
MHDA- 2009/04/03 09:00
PMCR- 2015/01/21
CRDT- 2009/02/10 09:00
PHST- 2009/02/10 09:00 [entrez]
PHST- 2009/02/10 09:00 [pubmed]
PHST- 2009/04/03 09:00 [medline]
PHST- 2015/01/21 00:00 [pmc-release]
AID - S0076-6879(08)03619-7 [pii]
AID - 10.1016/S0076-6879(08)03619-7 [doi]
PST - ppublish
SO  - Methods Enzymol. 2009;452:297-324. doi: 10.1016/S0076-6879(08)03619-7.