PMID- 19201263
OWN - NLM
STAT- MEDLINE
DCOM- 20090407
LR  - 20240109
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 877
IP  - 8-9
DP  - 2009 Mar 15
TI  - Simultaneous determination of 6-gingerol, 8-gingerol, 10-gingerol and 6-shogaol 
      in rat plasma by liquid chromatography-mass spectrometry: Application to 
      pharmacokinetics.
PG  - 671-9
LID - 10.1016/j.jchromb.2009.01.021 [doi]
AB  - A rapid high-performance liquid chromatography-mass spectrometry (HPLC-MS) method 
      was developed and validated for simultaneous quantification of 6-gingerol, 
      8-gingerol, 10-gingerol and 6-shogaol in rat plasma after oral administration of 
      ginger oleoresin. Plasma samples extracted with a liquid-liquid extraction 
      procedure were separated on an Agilent Zorbax StableBond-C(18) column (4.6 mm x 
      50 mm, 1.8 microm) and detected by MS with electrospray ionization interface in 
      positive selective ion monitoring (SIM) mode. Calibration curves (1/x(2) 
      weighted) offered satisfactory linearity (r(2)>0.995) in a wide linear range 
      (0.0104-13.0 microg/mL for 6-gingerol, 0.00357-4.46 microg/mL for 8-gingerol, 
      0.00920-11.5 microg/mL for 10-gingerol and 0.00738-9.22 microg/mL for 6-shogaol). 
      The lower limit of quantification (LLOQ) was in a range of 3.57-10.4 ng/mL. The 
      analytes and internal standard can be baseline separated within 6 min. Inter- and 
      intra-day assay variation was less than 15%. This developed method was 
      successfully applied to pharmacokinetic studies of ginger oleoresin after oral 
      administration to rats. Glucuronide of 6-gingerol was determined after 
      beta-glucuronidase hydrolysis for more information, and the intestinal 
      glucuronidation was further confirmed by comparison of plasma samples of hepatic 
      portal vein and femoral vein.
FAU - Wang, Wei
AU  - Wang W
AD  - Key Laboratory of Modern Chinese Medicines (China Pharmaceutical University), 
      Ministry of Education; Nanjing, 210009, China.
FAU - Li, Chang-Yin
AU  - Li CY
FAU - Wen, Xiao-Dong
AU  - Wen XD
FAU - Li, Ping
AU  - Li P
FAU - Qi, Lian-Wen
AU  - Qi LW
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090123
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Catechols)
RN  - 0 (Fatty Alcohols)
RN  - 925QK2Z900 (gingerol)
SB  - IM
MH  - Animals
MH  - Catechols/*blood/chemistry/*pharmacokinetics
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Fatty Alcohols/*blood/chemistry/*pharmacokinetics
MH  - Male
MH  - Mass Spectrometry/*methods
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2009/02/10 09:00
MHDA- 2009/04/08 09:00
CRDT- 2009/02/10 09:00
PHST- 2008/09/29 00:00 [received]
PHST- 2009/01/12 00:00 [revised]
PHST- 2009/01/16 00:00 [accepted]
PHST- 2009/02/10 09:00 [entrez]
PHST- 2009/02/10 09:00 [pubmed]
PHST- 2009/04/08 09:00 [medline]
AID - S1570-0232(09)00044-0 [pii]
AID - 10.1016/j.jchromb.2009.01.021 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Mar 15;877(8-9):671-9. doi: 
      10.1016/j.jchromb.2009.01.021. Epub 2009 Jan 23.