PMID- 19204092
OWN - NLM
STAT- MEDLINE
DCOM- 20090330
LR  - 20211020
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Print)
IS  - 0019-9567 (Linking)
VI  - 77
IP  - 4
DP  - 2009 Apr
TI  - Paracoccidioides brasiliensis vaccine formulations based on the gp43-derived P10 
      sequence and the Salmonella enterica FliC flagellin.
PG  - 1700-7
LID - 10.1128/IAI.01470-08 [doi]
AB  - Paracoccidioidomycosis (PCM) is a systemic granulomatous disease caused by the 
      dimorphic fungus Paracoccidioides brasiliensis. Anti-PCM vaccine formulations 
      based on the secreted fungal cell wall protein (gp43) or the derived P10 sequence 
      containing a CD4(+) T-cell-specific epitope have shown promising results. In the 
      present study, we evaluated new anti-PCM vaccine formulations based on the 
      intranasal administration of P. brasiliensis gp43 or the P10 peptide in 
      combination with the Salmonella enterica FliC flagellin, an innate immunity 
      agonist binding specifically to the Toll-like receptor 5, in a murine model. 
      BALB/c mice immunized with gp43 developed high-specific-serum immunoglobulin G1 
      responses and enhanced interleukin-4 (IL-4) and IL-10 levels. On the other hand, 
      mice immunized with recombinant purified flagellins genetically fused with P10 at 
      the central hypervariable domain, either flanked or not by two lysine residues, 
      or the synthetic P10 peptide admixed with purified FliC elicited a prevailing 
      Th1-type immune response based on lung cell-secreted type 1 cytokines. Mice 
      immunized with gp43 and FliC and intratracheally challenged with P. brasiliensis 
      yeast cells had increased fungal proliferation and lung tissue damage. In 
      contrast, mice immunized with the chimeric flagellins and particularly those 
      immunized with P10 admixed with FliC reduced P. brasiliensis growth and lung 
      damage. Altogether, these results indicate that S. enterica FliC flagellin 
      modulates the immune response to P. brasiliensis P10 antigen and represents a 
      promising alternative for the generation of anti-PCM vaccines.
FAU - Braga, Catarina J M
AU  - Braga CJ
AD  - Department of Microbiology-ICB, University of Sao Paulo, Av. Prof. Lineu Prestes 
      1374, Sao Paulo, SP 05008-000, Brazil.
FAU - Rittner, Glauce M G
AU  - Rittner GM
FAU - Munoz Henao, Julian E
AU  - Munoz Henao JE
FAU - Teixeira, Aline F
AU  - Teixeira AF
FAU - Massis, Liliana M
AU  - Massis LM
FAU - Sbrogio-Almeida, Maria E
AU  - Sbrogio-Almeida ME
FAU - Taborda, Carlos P
AU  - Taborda CP
FAU - Travassos, Luiz R
AU  - Travassos LR
FAU - Ferreira, Luis C S
AU  - Ferreira LC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090209
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (43 kDa protein, Paracoccidioides)
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Antigens, Fungal)
RN  - 0 (Cytokines)
RN  - 0 (Fungal Proteins)
RN  - 0 (Fungal Vaccines)
RN  - 0 (Glycoproteins)
RN  - 0 (Peptides)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 12777-81-0 (Flagellin)
SB  - IM
MH  - Animals
MH  - Antibodies, Bacterial/blood
MH  - Antibody Specificity
MH  - *Antigens, Fungal/chemistry/genetics/immunology
MH  - Cytokines/metabolism
MH  - Disease Models, Animal
MH  - *Flagellin/genetics/immunology/metabolism
MH  - *Fungal Proteins/chemistry/genetics/immunology
MH  - *Fungal Vaccines/administration & dosage/genetics/immunology
MH  - *Glycoproteins/chemistry/genetics/immunology
MH  - Humans
MH  - Immunization
MH  - Lung/immunology/microbiology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Paracoccidioides/*immunology/pathogenicity
MH  - Paracoccidioidomycosis/immunology/microbiology/*prevention & control
MH  - *Peptides/chemical synthesis/genetics/immunology
MH  - Recombinant Fusion Proteins/genetics/immunology/metabolism
MH  - Salmonella enterica/genetics/immunology/metabolism
PMC - PMC2663153
EDAT- 2009/02/11 09:00
MHDA- 2009/03/31 09:00
PMCR- 2009/10/01
CRDT- 2009/02/11 09:00
PHST- 2009/02/11 09:00 [entrez]
PHST- 2009/02/11 09:00 [pubmed]
PHST- 2009/03/31 09:00 [medline]
PHST- 2009/10/01 00:00 [pmc-release]
AID - IAI.01470-08 [pii]
AID - 1470-08 [pii]
AID - 10.1128/IAI.01470-08 [doi]
PST - ppublish
SO  - Infect Immun. 2009 Apr;77(4):1700-7. doi: 10.1128/IAI.01470-08. Epub 2009 Feb 9.