PMID- 19207928
OWN - NLM
STAT- MEDLINE
DCOM- 20090331
LR  - 20090211
IS  - 0931-2668 (Print)
IS  - 0931-2668 (Linking)
VI  - 126
IP  - 1
DP  - 2009 Feb
TI  - The pig CART (cocaine- and amphetamine-regulated transcript) gene and association 
      of its microsatellite polymorphism with production traits.
PG  - 37-42
LID - 10.1111/j.1439-0388.2008.00750.x [doi]
AB  - The cocaine- and amphetamine-regulated transcript (CART) gene is a candidate gene 
      that may affect performance and body composition traits in the pig. The purpose 
      of this study was to establish the chromosomal localization and genomic sequence 
      of the porcine CART gene, search for polymorphism and analyse its phenotypic 
      effect in 644 pigs representing two breeds, Polish Large White (PLW) and Polish 
      Landrace (PL), and a synthetic line 990 (L990). The CART gene was fluorescence in 
      situ hybridization (FISH)-mapped to the chromosome 16q21. The 1878 bp DNA 
      fragment covering three exons, two introns and the 5' flanking region was 
      sequenced and analysed. A new A/G single nucleotide polymorphism (SNP) at 
      position -238 bp was found. The coding sequence was conserved between porcine and 
      human CART genes. Previously unknown short tandem repeat polymorphism 
      (CA)(2)(CG)(n)(CA)(n) was identified in intron 2. Three alleles 251, 253 and 259 
      bp were found. The 251-bp allele was predominant in all the analysed populations 
      of pigs, whereas the 253-bp allele occurred with the lowest frequency. The 
      statistical analysis revealed significant allelic additive effects on meat 
      content in carcass (p < 0.05) and abdominal fat weight (p < 0.01) in PLW, and 
      meat content in carcass (p < 0.05) and backfat thickness (p < 0.05) in PL. Our 
      study confirmed that chromosome region harbouring the CART gene is a promising 
      quantitative trait loci for pig production traits.
FAU - Stachowiak, M
AU  - Stachowiak M
AD  - Department of Genetics and Animal Breeding, University of Life Sciences in 
      Poznan, Poland.
FAU - Cieslak, J
AU  - Cieslak J
FAU - Skorczyk, A
AU  - Skorczyk A
FAU - Nowakowska, J
AU  - Nowakowska J
FAU - Szczerbal, I
AU  - Szczerbal I
FAU - Szydlowski, M
AU  - Szydlowski M
FAU - Switonski, M
AU  - Switonski M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - J Anim Breed Genet
JT  - Journal of animal breeding and genetics = Zeitschrift fur Tierzuchtung und 
      Zuchtungsbiologie
JID - 100955807
RN  - 0 (DNA Primers)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (cocaine- and amphetamine-regulated transcript protein)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Body Composition/*genetics
MH  - Breeding/*methods
MH  - Chromosomes, Mammalian/*genetics
MH  - DNA Primers/genetics
MH  - Gene Frequency
MH  - In Situ Hybridization, Fluorescence/veterinary
MH  - *Meat
MH  - Microsatellite Repeats/genetics
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/*genetics
MH  - Phylogeny
MH  - Polymorphism, Single Nucleotide/genetics
MH  - Sequence Analysis, DNA/veterinary
MH  - Sus scrofa/*genetics
EDAT- 2009/02/12 09:00
MHDA- 2009/04/01 09:00
CRDT- 2009/02/12 09:00
PHST- 2009/02/12 09:00 [entrez]
PHST- 2009/02/12 09:00 [pubmed]
PHST- 2009/04/01 09:00 [medline]
AID - JBG750 [pii]
AID - 10.1111/j.1439-0388.2008.00750.x [doi]
PST - ppublish
SO  - J Anim Breed Genet. 2009 Feb;126(1):37-42. doi: 10.1111/j.1439-0388.2008.00750.x.