PMID- 19208854
OWN - NLM
STAT- MEDLINE
DCOM- 20090521
LR  - 20220409
IS  - 0193-1849 (Print)
IS  - 0193-1849 (Linking)
VI  - 296
IP  - 4
DP  - 2009 Apr
TI  - Berberine suppresses proinflammatory responses through AMPK activation in 
      macrophages.
PG  - E955-64
LID - 10.1152/ajpendo.90599.2008 [doi]
AB  - Berberine (BBR) has been shown to improve several metabolic disorders, such as 
      obesity, type 2 diabetes, and dyslipidemia, by stimulating AMP-activated protein 
      kinase (AMPK). However, the effects of BBR on proinflammatory responses in 
      macrophages are poorly understood. Here we show that BBR represses 
      proinflammatory responses through AMPK activation in macrophages. In adipose 
      tissue of obese db/db mice, BBR treatment significantly downregulated the 
      expression of proinflammatory genes such as TNF-alpha, IL-1beta, IL-6, monocyte 
      chemoattractant protein-1 (MCP-1), inducible nitric oxide synthase (iNOS), and 
      cyclooxygenase-2 (COX-2). Consistently, BBR inhibited LPS-induced expression of 
      proinflammatory genes including IL-1beta, IL-6, iNOS, MCP-1, COX-2, and matrix 
      metalloprotease-9 in peritoneal macrophages and RAW 264.7 cells. Upon various 
      proinflammatory signals including LPS, free fatty acids, and hydrogen peroxide, 
      BBR suppressed the phosphorylation of MAPKs, such as p38, ERK, and JNK, and the 
      level of reactive oxygen species in macrophages. Moreover, these inhibitory 
      effects of BBR on proinflammatory responses were abolished by AMPK inhibition via 
      either compound C, an AMPK inhibitor, or dominant-negative AMPK, implying that 
      BBR would downregulate proinflammatory responses in macrophages via AMPK 
      stimulation.
FAU - Jeong, Hyun Woo
AU  - Jeong HW
AD  - Institute of Molecular Biology and Genetics, Department of Biological Sciences, 
      Seoul National University, San 56-1, Sillim-Dong, Kwanak-Gu, Seoul 151-742, 
      Korea.
FAU - Hsu, Kuan Chi
AU  - Hsu KC
FAU - Lee, Joo-Won
AU  - Lee JW
FAU - Ham, Mira
AU  - Ham M
FAU - Huh, Jin Young
AU  - Huh JY
FAU - Shin, Hyun Jung
AU  - Shin HJ
FAU - Kim, Woo Sik
AU  - Kim WS
FAU - Kim, Jae Bum
AU  - Kim JB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090210
PL  - United States
TA  - Am J Physiol Endocrinol Metab
JT  - American journal of physiology. Endocrinology and metabolism
JID - 100901226
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Receptors, Leptin)
RN  - 0I8Y3P32UF (Berberine)
RN  - EC 2.7.4.3 (Adenylate Kinase)
SB  - IM
MH  - 3T3-L1 Cells
MH  - Adenylate Kinase/metabolism/*physiology
MH  - Adipose Tissue, White/drug effects/pathology
MH  - Animals
MH  - Anti-Inflammatory Agents/pharmacology/therapeutic use
MH  - Berberine/*pharmacology/therapeutic use
MH  - Cells, Cultured
MH  - Drug Evaluation, Preclinical
MH  - Gene Expression Regulation/drug effects
MH  - Inflammation/drug therapy/genetics/pathology
MH  - Inflammation Mediators/*antagonists & inhibitors
MH  - Macrophages/*drug effects/enzymology/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Obese
MH  - Mice, Transgenic
MH  - Receptors, Leptin/genetics
EDAT- 2009/02/12 09:00
MHDA- 2009/05/22 09:00
CRDT- 2009/02/12 09:00
PHST- 2009/02/12 09:00 [entrez]
PHST- 2009/02/12 09:00 [pubmed]
PHST- 2009/05/22 09:00 [medline]
AID - 90599.2008 [pii]
AID - 10.1152/ajpendo.90599.2008 [doi]
PST - ppublish
SO  - Am J Physiol Endocrinol Metab. 2009 Apr;296(4):E955-64. doi: 
      10.1152/ajpendo.90599.2008. Epub 2009 Feb 10.