PMID- 19211022
OWN - NLM
STAT- MEDLINE
DCOM- 20090310
LR  - 20090304
IS  - 1090-2449 (Electronic)
IS  - 0014-4894 (Linking)
VI  - 121
IP  - 4
DP  - 2009 Apr
TI  - The role of liposome charge on immune response generated in BALB/c mice immunized 
      with recombinant major surface glycoprotein of Leishmania (rgp63).
PG  - 362-9
LID - 10.1016/j.exppara.2008.12.015 [doi]
AB  - Liposomes as a lipid-based system have been shown to be an effective adjuvant 
      formulation. In this study, the role of liposome charge in induction of a Th1 
      type of immune response and protection against leishmaniasis in BALB/c mice was 
      studied. Liposomes containing rgp63 were prepared by Dehydration-Rehydration 
      Vesicle (DRV) method. Neutral liposomes consisted of 
      dipalmitoylphosphatidylcholine and cholesterol. Positively and negatively charged 
      liposomes were prepared by adding dimethyldioctadecylammonium bromide (DDAB) or 
      dicetyl phosphate (DCP) to the neutral liposome formulation, respectively. Female 
      BALB/c mice were immunized subcutaneously with negatively, positively charged or 
      neutral liposomes encapsulated with rgp63, rgp63 in soluble form or PBS, three 
      times in 3week intervals. The extent of protection and type of immune response 
      generated were studied in different groups of mice. The group of mice immunized 
      with rgp63 encapsulated in neutral liposomes showed a significantly (P<0.01) 
      smaller footpad swelling upon challenge with Leishmania major compared with 
      positively or negatively charged liposomes. The mice immunized with neutral 
      liposomes also showed a significantly (P<0.01) the lowest splenic parasite 
      burden, the highest IgG2a/IgG1 ratio and IFN-gamma production and the lowest IL-4 
      level compared to the other groups. The results indicated that a Th1 type of 
      immune response was induced in mice immunized with neutral liposomes more 
      efficiently than positively charged liposomes and conversely negatively charged 
      liposomes induced a Th2 type of immune response.
FAU - Badiee, Ali
AU  - Badiee A
AD  - School of Pharmacy, Biotechnology Research Center and Pharmaceutical Research 
      Center, Mashhad University of Medical Sciences, Vakil-Abad Blvd, P.O. Box 
      91775-1365, Mashhad, Khorasan, Iran.
FAU - Jaafari, Mahmoud R
AU  - Jaafari MR
FAU - Khamesipour, Ali
AU  - Khamesipour A
FAU - Samiei, Afshin
AU  - Samiei A
FAU - Soroush, Dina
AU  - Soroush D
FAU - Kheiri, Masoumeh Tavassoti
AU  - Kheiri MT
FAU - Barkhordari, Farzaneh
AU  - Barkhordari F
FAU - McMaster, W Robert
AU  - McMaster WR
FAU - Mahboudi, Fereidoun
AU  - Mahboudi F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090101
PL  - United States
TA  - Exp Parasitol
JT  - Experimental parasitology
JID - 0370713
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Antibodies, Protozoan)
RN  - 0 (Antigens, Protozoan)
RN  - 0 (Drug Carriers)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Liposomes)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Protozoan Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 207137-56-2 (Interleukin-4)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adjuvants, Immunologic
MH  - Animals
MH  - Antibodies, Protozoan/biosynthesis/blood
MH  - Antigens, Protozoan/administration & dosage/immunology
MH  - Drug Carriers
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Female
MH  - Immunization/*methods
MH  - Immunoglobulin G/biosynthesis/blood
MH  - Interferon-gamma/biosynthesis
MH  - Interleukin-4/biosynthesis
MH  - Leishmania major/*immunology
MH  - Leishmaniasis, Cutaneous/immunology/prevention & control
MH  - Liposomes/immunology/*metabolism
MH  - Membrane Glycoproteins/administration & dosage/*immunology
MH  - Mice
MH  - Protozoan Proteins/administration & dosage/*immunology
MH  - Recombinant Proteins/administration & dosage/immunology
MH  - Spleen/cytology/immunology/parasitology
MH  - Th1 Cells/immunology
EDAT- 2009/02/13 09:00
MHDA- 2009/03/11 09:00
CRDT- 2009/02/13 09:00
PHST- 2008/08/07 00:00 [received]
PHST- 2008/11/30 00:00 [revised]
PHST- 2008/12/22 00:00 [accepted]
PHST- 2009/02/13 09:00 [entrez]
PHST- 2009/02/13 09:00 [pubmed]
PHST- 2009/03/11 09:00 [medline]
AID - S0014-4894(08)00342-1 [pii]
AID - 10.1016/j.exppara.2008.12.015 [doi]
PST - ppublish
SO  - Exp Parasitol. 2009 Apr;121(4):362-9. doi: 10.1016/j.exppara.2008.12.015. Epub 
      2009 Jan 1.