PMID- 19211265
OWN - NLM
STAT- MEDLINE
DCOM- 20100813
LR  - 20211020
IS  - 1879-1336 (Electronic)
IS  - 1054-8807 (Print)
IS  - 1054-8807 (Linking)
VI  - 19
IP  - 3
DP  - 2010 May-Jun
TI  - Creation, validation, and quantitative analysis of protein expression in vascular 
      tissue microarrays.
PG  - 136-46
LID - 10.1016/j.carpath.2008.12.007 [doi]
AB  - BACKGROUND: Tissue microarrays (TMAs) are collections of multiple tissue cores 
      placed in parallel in a single acceptor block and traditionally used to 
      investigate protein expression in neoplastic tissues. We validated the use of 
      TMAs to investigate protein expression in vascular segments. METHODS: Vascular 
      tissues were collected from 100 adult subjects undergoing autopsy. A diverse set 
      of vessels were harvested and arrayed over 17 TMAs. A total of 1377 unique 
      tissues, each with a 1.5-mm feature size, were analyzed using histochemical and 
      immunohistochemical (IHC) diaminobenzidine (DAB) methods. RESULTS: 
      Histomorphometric analysis of vascular disease demonstrated the TMA features 
      captured the majority of the vascular alterations (intimal hyperplasia and 
      atherosclerosis) seen in the original blood vessel section. Measurements of IHC 
      staining intensity based on color deconvolution were used to quantify antigen 
      abundance in defined regions of interest (ROI). Validation was performed using 
      antibodies to connective tissue growth factor (CTGF), receptor for advanced 
      glycation end products (AGER/RAGE), and matrix metalloproteinase 3 (MMP-3). IHC 
      staining was highly correlated between duplicate features from the same vascular 
      site over these three proteins. CONCLUSION: This study validates the use of TMA 
      technology to investigate the vascular wall utilizing staining intensity data.
CI  - Copyright 2010 Elsevier Inc. All rights reserved.
FAU - Halushka, Marc K
AU  - Halushka MK
AD  - Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, 
      MD, USA. mhalush1@jhmi.edu
FAU - Cornish, Toby C
AU  - Cornish TC
FAU - Lu, Jie
AU  - Lu J
FAU - Selvin, Steve
AU  - Selvin S
FAU - Selvin, Elizabeth
AU  - Selvin E
LA  - eng
GR  - K01 DK076595-03/DK/NIDDK NIH HHS/United States
GR  - K01 DK076595-04/DK/NIDDK NIH HHS/United States
GR  - K01 DK076595/DK/NIDDK NIH HHS/United States
GR  - K01 DK076595-01/DK/NIDDK NIH HHS/United States
GR  - K01 DK076595-02/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
DEP - 20090211
PL  - United States
TA  - Cardiovasc Pathol
JT  - Cardiovascular pathology : the official journal of the Society for Cardiovascular 
      Pathology
JID - 9212060
RN  - 0 (Proteins)
SB  - IM
MH  - Autopsy
MH  - Blood Vessels/metabolism/*pathology
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Male
MH  - Middle Aged
MH  - Proteins/analysis/*metabolism
MH  - Tissue Array Analysis/*methods
MH  - Vascular Diseases/diagnosis/metabolism/*pathology
PMC - PMC2866781
MID - NIHMS154414
EDAT- 2009/02/13 09:00
MHDA- 2010/08/14 06:00
PMCR- 2011/05/01
CRDT- 2009/02/13 09:00
PHST- 2008/08/19 00:00 [received]
PHST- 2008/11/21 00:00 [revised]
PHST- 2008/12/16 00:00 [accepted]
PHST- 2009/02/13 09:00 [entrez]
PHST- 2009/02/13 09:00 [pubmed]
PHST- 2010/08/14 06:00 [medline]
PHST- 2011/05/01 00:00 [pmc-release]
AID - S1054-8807(08)00188-9 [pii]
AID - 10.1016/j.carpath.2008.12.007 [doi]
PST - ppublish
SO  - Cardiovasc Pathol. 2010 May-Jun;19(3):136-46. doi: 10.1016/j.carpath.2008.12.007. 
      Epub 2009 Feb 11.