PMID- 19211896
OWN - NLM
STAT- MEDLINE
DCOM- 20090306
LR  - 20211020
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 29
IP  - 6
DP  - 2009 Feb 11
TI  - Viral vector-mediated overexpression of estrogen receptor-alpha in striatum 
      enhances the estradiol-induced motor activity in female rats and 
      estradiol-modulated GABA release.
PG  - 1897-903
LID - 10.1523/JNEUROSCI.4647-08.2009 [doi]
AB  - Classical estrogen receptor-signaling mechanisms involve estradiol binding to 
      intracellular nuclear receptors [estrogen receptor-alpha (ERalpha) and estrogen 
      receptor-beta (ERbeta)] to promote changes in protein expression. Estradiol can 
      also exert effects within seconds to minutes, however, a timescale incongruent 
      with genomic signaling. In the brain, estradiol rapidly potentiates stimulated 
      dopamine release in the striatum of female rats and enhances spontaneous 
      rotational behavior. Furthermore, estradiol rapidly attenuates the K(+)-evoked 
      increase of GABA in dialysate. We hypothesize that these rapid effects of 
      estradiol in the striatum are mediated by ERalpha located on the membrane of 
      medium spiny GABAergic neurons. This experiment examined whether overexpression 
      of ERalpha in the striatum would enhance the effect of estradiol on rotational 
      behavior and the K(+)-evoked increase in GABA in dialysate. Ovariectomized female 
      rats were tested for rotational behavior or underwent microdialysis experiments 
      after unilateral intrastriatal injections of a recombinant adeno-associated virus 
      (AAV) containing the human ERalpha cDNA (AAV.ERalpha) into the striatum; controls 
      received either the same vector into areas outside the striatum or an AAV 
      containing the human alkaline phosphatase gene into the striatum (AAV.ALP). 
      Animals that received AAV.ERalpha in the striatum exhibited significantly greater 
      estradiol-induced contralateral rotations compared with controls and exhibited 
      behavioral sensitization of contralateral rotations induced by a low-dose of 
      amphetamine. ERalpha overexpression also enhanced the inhibitory effect of 
      estradiol on K(+)-evoked GABA release suggesting that disinhibition of dopamine 
      release from terminals in the striatum resulted in the enhanced rotational 
      behavior.
FAU - Schultz, Kristin N
AU  - Schultz KN
AD  - Molecular and Behavioral Neuroscience Institute and Department of Chemistry, 
      University of Michigan, Ann Arbor, Michigan 48109, USA.
FAU - von Esenwein, Silke A
AU  - von Esenwein SA
FAU - Hu, Ming
AU  - Hu M
FAU - Bennett, Amy L
AU  - Bennett AL
FAU - Kennedy, Robert T
AU  - Kennedy RT
FAU - Musatov, Sergei
AU  - Musatov S
FAU - Toran-Allerand, C Dominique
AU  - Toran-Allerand CD
FAU - Kaplitt, Michael G
AU  - Kaplitt MG
FAU - Young, Larry J
AU  - Young LJ
FAU - Becker, Jill B
AU  - Becker JB
LA  - eng
GR  - DA12677/DA/NIDA NIH HHS/United States
GR  - NS048141/NS/NINDS NIH HHS/United States
GR  - R01 NS048141-02/NS/NINDS NIH HHS/United States
GR  - R37 EB003320/EB/NIBIB NIH HHS/United States
GR  - R01 DA012677-06/DA/NIDA NIH HHS/United States
GR  - R01 DA012677-05/DA/NIDA NIH HHS/United States
GR  - R01 NS048141/NS/NINDS NIH HHS/United States
GR  - R01 DA012677/DA/NIDA NIH HHS/United States
GR  - R01 DA012677-08/DA/NIDA NIH HHS/United States
GR  - T32 DA007267/DA/NIDA NIH HHS/United States
GR  - R01 NS048141-03/NS/NINDS NIH HHS/United States
GR  - R01 DA012677-07/DA/NIDA NIH HHS/United States
GR  - R01 NS048141-04/NS/NINDS NIH HHS/United States
GR  - R01 NS048141-01/NS/NINDS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Estrogen Receptor alpha)
RN  - 4TI98Z838E (Estradiol)
RN  - 56-12-2 (gamma-Aminobutyric Acid)
SB  - IM
MH  - Animals
MH  - Corpus Striatum/*metabolism/physiology/*virology
MH  - Dependovirus/genetics
MH  - Estradiol/genetics/*physiology
MH  - Estrogen Receptor alpha/administration & dosage/*biosynthesis/*genetics
MH  - Female
MH  - Gene Expression Regulation/*physiology
MH  - Genetic Vectors/administration & dosage/genetics
MH  - Humans
MH  - Motor Activity/genetics/*physiology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sexual Behavior/physiology
MH  - gamma-Aminobutyric Acid/*metabolism
PMC - PMC2655639
MID - NIHMS93860
EDAT- 2009/02/13 09:00
MHDA- 2009/03/07 09:00
PMCR- 2009/08/11
CRDT- 2009/02/13 09:00
PHST- 2009/02/13 09:00 [entrez]
PHST- 2009/02/13 09:00 [pubmed]
PHST- 2009/03/07 09:00 [medline]
PHST- 2009/08/11 00:00 [pmc-release]
AID - 29/6/1897 [pii]
AID - 3452044 [pii]
AID - 10.1523/JNEUROSCI.4647-08.2009 [doi]
PST - ppublish
SO  - J Neurosci. 2009 Feb 11;29(6):1897-903. doi: 10.1523/JNEUROSCI.4647-08.2009.