PMID- 19212757
OWN - NLM
STAT- MEDLINE
DCOM- 20091001
LR  - 20171116
IS  - 1432-0738 (Electronic)
IS  - 0340-5761 (Linking)
VI  - 83
IP  - 7
DP  - 2009 Jul
TI  - Investigation of the sensitising and cross-sensitising potential of textile dyes 
      and beta-lactam antibiotics using a biphasic mice local lymph node assay.
PG  - 691-9
LID - 10.1007/s00204-009-0407-1 [doi]
AB  - We used a modified protocol of the murine local lymph node assay (LLNA) to study 
      the cross-sensitising potential of (a) textile dye disperse yellow 3 and its 
      metabolite 2-amino-p-cresol, (b) two antibiotics, penicillin G and cefotiam. The 
      test substances were applied in a biphasic manner, i.e. first on the shaved skin 
      of the back followed by application on the dorsal side of the ears after 2 weeks. 
      The end-points analysed included thickness and weight of an ear-biopsy, weight 
      and cell number of the draining lymph node, and lymphocyte cell surface markers 
      analysed by flow-cytometry. Disperse yellow 3 and its metabolite significantly 
      altered the various end-points at both the tested concentrations (0.5 and 1%), 
      thus demonstrating the sensitising potential of the two substances. The 
      cross-sensitisation study showed significant modulation in the tested variables 
      in the treated group as compared to the control, signifying cross-sensitisation 
      potential of the two substances. Penicillin G and cefotiam showed significant 
      changes in various end-points, pointing towards their sensitising potential. 
      However, even at 50% concentration of the beta-lactams no significant change in 
      any end-point indicating absence of cross-reactivity of the antibiotics was 
      noticed. We conclude that a biphasic, modified protocol of the LLNA is a suitable 
      approach to test for a cross-reactivity potential of two related compounds.
FAU - Ahuja, Varun
AU  - Ahuja V
AD  - Department of Toxicology, Institute of Clinical Pharmacology and Toxicology, 
      Charite Medical University, Campus Benjamin Franklin, Berlin, Germany.
FAU - Schreiber, Clemens
AU  - Schreiber C
FAU - Platzek, Thomas
AU  - Platzek T
FAU - Stahlmann, Ralf
AU  - Stahlmann R
LA  - eng
PT  - Journal Article
DEP - 20090211
PL  - Germany
TA  - Arch Toxicol
JT  - Archives of toxicology
JID - 0417615
RN  - 0 (Allergens)
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Azo Compounds)
RN  - 0 (Biomarkers)
RN  - 0 (Coloring Agents)
RN  - 0 (Cresols)
RN  - 0 (beta-Lactams)
RN  - 0890872E2E (Dispersion Yellow 3)
SB  - IM
MH  - Allergens/metabolism/*toxicity
MH  - Animals
MH  - Anti-Bacterial Agents/*toxicity
MH  - Azo Compounds/metabolism/toxicity
MH  - B-Lymphocytes/drug effects
MH  - Biomarkers/analysis
MH  - Coloring Agents/metabolism/*toxicity
MH  - Cresols/toxicity
MH  - Cross Reactions
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - *Local Lymph Node Assay
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Skin/drug effects/metabolism
MH  - T-Lymphocytes/drug effects
MH  - *Textiles
MH  - beta-Lactams/*toxicity
EDAT- 2009/02/13 09:00
MHDA- 2009/10/02 06:00
CRDT- 2009/02/13 09:00
PHST- 2008/09/09 00:00 [received]
PHST- 2009/01/15 00:00 [accepted]
PHST- 2009/02/13 09:00 [entrez]
PHST- 2009/02/13 09:00 [pubmed]
PHST- 2009/10/02 06:00 [medline]
AID - 10.1007/s00204-009-0407-1 [doi]
PST - ppublish
SO  - Arch Toxicol. 2009 Jul;83(7):691-9. doi: 10.1007/s00204-009-0407-1. Epub 2009 Feb 
      11.