PMID- 19214565
OWN - NLM
STAT- MEDLINE
DCOM- 20090818
LR  - 20211020
IS  - 1432-2307 (Electronic)
IS  - 0945-6317 (Linking)
VI  - 454
IP  - 4
DP  - 2009 Apr
TI  - Apoptosis of interstitial cells of Cajal, smooth muscle cells, and enteric 
      neurons induced by intestinal ischemia and reperfusion injury in adult guinea 
      pigs.
PG  - 401-9
LID - 10.1007/s00428-009-0739-5 [doi]
AB  - This study aimed at evaluating whether apoptosis of interstitial cells of Cajal 
      (ICC), smooth muscle cells (SMC), and enteric neurons was involved in a guinea 
      pig model of intestinal ischemia and reperfusion injury. The small intestinal 
      segments were resected at either 6 (I60/R6h) and 12 h (I60/R12h) or 7 (I60/R7d) 
      to 14 (I60/R14d) days after 60 min intestinal ischemia in the adult guinea pigs 
      and studied by immunohistochemistry with anti-Kit, 5-bromo-2'-deoxyuridine 
      (BrdU), alpha-smooth muscle actin, vimentin, and beta-tublin III antibodies. 
      Also, apoptosis was tested by terminal deoxynucleotidyl transferase-mediated dUTP 
      nick end labeling (TUNEL) method. In the I60/R12h injury, there was a 
      approximately 50% decrease of Kit+ cells in cell numbers at the level of 
      myenteric plexus and a number of Kit-/vimentin-positive cells were labeled by 
      TUNEL. Also, a few SMC and enteric neurons were TUNEL positive. The Kit+ ICC 
      recovered to normal and a number of Kit-/BrdU-double-positive cells were observed 
      in the I60/R14d group. Our results indicated that the intestinal I/R injury could 
      lead to apoptosis of ICC, SMC, and enteric neurons which may contribute to the 
      gastrointestinal motility disorders, and proliferation was involved in the 
      recovery of ICC.
FAU - Mei, Feng
AU  - Mei F
AD  - Department of Histology and Embryology, Third Military Medical University, 
      Chongqing 400038, China. thethirdmilitary@sohu.com
FAU - Guo, Sheng
AU  - Guo S
FAU - He, Yang-tao
AU  - He YT
FAU - Zhu, Jiang
AU  - Zhu J
FAU - Zhou, De-shan
AU  - Zhou DS
FAU - Niu, Jian-qin
AU  - Niu JQ
FAU - Wang, Han-zhi
AU  - Wang HZ
FAU - Tian, Yan-ping
AU  - Tian YP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090212
PL  - Germany
TA  - Virchows Arch
JT  - Virchows Archiv : an international journal of pathology
JID - 9423843
RN  - 0 (Actins)
RN  - 0 (Tubulin)
RN  - 0 (Vimentin)
SB  - IM
MH  - Actins/metabolism
MH  - Animals
MH  - Apoptosis/*physiology
MH  - Fluorescent Antibody Technique
MH  - Guinea Pigs
MH  - Immunohistochemistry
MH  - In Situ Nick-End Labeling
MH  - Intestinal Diseases/metabolism/*pathology
MH  - Intestines/blood supply/innervation/pathology
MH  - Myenteric Plexus/metabolism/pathology
MH  - Myocytes, Smooth Muscle/metabolism/*pathology
MH  - Neurons/metabolism/*pathology
MH  - Reperfusion Injury/metabolism/*pathology
MH  - Tubulin/metabolism
MH  - Vimentin/metabolism
EDAT- 2009/02/14 09:00
MHDA- 2009/08/19 09:00
CRDT- 2009/02/14 09:00
PHST- 2008/08/06 00:00 [received]
PHST- 2009/01/18 00:00 [accepted]
PHST- 2009/01/17 00:00 [revised]
PHST- 2009/02/14 09:00 [entrez]
PHST- 2009/02/14 09:00 [pubmed]
PHST- 2009/08/19 09:00 [medline]
AID - 10.1007/s00428-009-0739-5 [doi]
PST - ppublish
SO  - Virchows Arch. 2009 Apr;454(4):401-9. doi: 10.1007/s00428-009-0739-5. Epub 2009 
      Feb 12.