PMID- 19214728
OWN - NLM
STAT- MEDLINE
DCOM- 20090908
LR  - 20211020
IS  - 1572-994X (Electronic)
IS  - 0920-8569 (Linking)
VI  - 38
IP  - 3
DP  - 2009 Jun
TI  - A multiplex PCR for rapid and simultaneous detection of porcine circovirus type 
      2, porcine parvovirus, porcine pseudorabies virus, and porcine reproductive and 
      respiratory syndrome virus in clinical specimens.
PG  - 392-7
LID - 10.1007/s11262-009-0333-6 [doi]
AB  - A multiplex PCR (mPCR) assay was developed and evaluated for its ability to 
      simultaneously detect multiple viral infections of swine. Specific primers were 
      designed for each of the following four DNA or RNA viruses: porcine circovirus 
      type 2 (PCV2), porcine parvovirus (PPV), pseudorabies virus (PRV), and porcine 
      reproductive and respiratory syndrome virus (PRRSV). Each target produced a 
      specific amplicon with a size of 353 bp (PCV2), 271 bp (PPV), 194 bp (PRV), or 
      434 bp (PRRSV). The assay was sensitive and specific in detecting each target 
      agent in composite cell cultures and clinical specimens. Results from mPCR were 
      confirmed by PCR for individual viruses and by virus isolation. In conclusion, 
      the mPCR has the potential to be useful for routine molecular diagnosis and 
      epidemiology.
FAU - Yue, Fengxiong
AU  - Yue F
AD  - Swine Infectious Disease Division, State Key Laboratory of Veterinary 
      Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of 
      Agricultural Sciences, Heilongjiang, People's Republic of China.
FAU - Cui, Shangjin
AU  - Cui S
FAU - Zhang, Chaofan
AU  - Zhang C
FAU - Yoon, Kyoung-Jin
AU  - Yoon KJ
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090212
PL  - United States
TA  - Virus Genes
JT  - Virus genes
JID - 8803967
RN  - 0 (DNA Primers)
SB  - IM
MH  - Animals
MH  - Circovirus/genetics/*isolation & purification
MH  - DNA Primers/genetics
MH  - Herpesvirus 1, Suid/genetics/*isolation & purification
MH  - Parvovirus, Porcine/genetics/*isolation & purification
MH  - Polymerase Chain Reaction/*methods
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*isolation & 
      purification
MH  - Sensitivity and Specificity
MH  - Swine
MH  - Swine Diseases/*diagnosis/virology
MH  - Virus Diseases/diagnosis/*veterinary
EDAT- 2009/02/14 09:00
MHDA- 2009/09/09 06:00
CRDT- 2009/02/14 09:00
PHST- 2008/08/17 00:00 [received]
PHST- 2009/01/26 00:00 [accepted]
PHST- 2009/02/14 09:00 [entrez]
PHST- 2009/02/14 09:00 [pubmed]
PHST- 2009/09/09 06:00 [medline]
AID - 10.1007/s11262-009-0333-6 [doi]
PST - ppublish
SO  - Virus Genes. 2009 Jun;38(3):392-7. doi: 10.1007/s11262-009-0333-6. Epub 2009 Feb 
      12.