PMID- 19215652
OWN - NLM
STAT- MEDLINE
DCOM- 20090407
LR  - 20131121
IS  - 0003-7028 (Print)
IS  - 0003-7028 (Linking)
VI  - 63
IP  - 2
DP  - 2009 Feb
TI  - Tuning the surface-enhanced Raman scattering effect to different molecular groups 
      by switching the silver colloid solution pH.
PG  - 214-23
LID - 10.1366/000370209787391987 [doi]
AB  - Silver colloids were produced for surface-enhanced Raman scattering (SERS) 
      experiments using hydroxylamine hydrochloride as the reduction agent. The roles 
      of hydroxylamine hydrochloride and bulk solution pH values in the formation of 
      functional groups on the surface of silver colloids and in determining the 
      dimensions of silver colloids were examined using Raman, Fourier transform 
      infrared (FT-IR) and ultraviolet-visible (UV-Vis) spectroscopy, atomic force 
      microscopy (AFM), and zeta-size measurements. The spectrum of hydroxylamine 
      hydrochloride reduced silver colloids was compared with the spectrum of sodium 
      borohydride reduced colloids. The effect of colloid solution pH on SERS results 
      was demonstrated using analyte molecules with biological significance, such as 
      ribonucleic acid, egg albumin, L-alpha-phosphatidylcholine, and glucose. In 
      general, it was shown that at high pH values the SERS effect was more pronounced 
      due to the surface functional groups and colloid dimensions, and sharp, high 
      spectral intensity values were obtained. At low pH values, protonation and rapid 
      aggregation of colloids occurred and the surface chemistry was different. 
      Depending on the analyte, bands were shifted, broadened, and/or the enhancement 
      effect was reduced. Using Pseudomonas aeruginosa PAO1 and Streptococcus mutans it 
      was also shown that by changing the solution bulk pH value, it was possible to 
      enhance the response from different molecular groups in the bacteria and obtain 
      different spectra from the same bacteria strain and the process was reversible. 
      It was concluded that it is possible to produce site- or molecule-specific metal 
      colloids and to tune the SERS effect to certain functional groups of analytes by 
      means of the pH of colloidal suspension.
FAU - Kazanci, Murat
AU  - Kazanci M
AD  - Department of Chemistry, St. Francis Xavier University, Antigonish, NS, Canada, 
      B2G 2W5.
FAU - Schulte, Jean Pascal
AU  - Schulte JP
FAU - Douglas, Colin
AU  - Douglas C
FAU - Fratzl, Peter
AU  - Fratzl P
FAU - Pink, David
AU  - Pink D
FAU - Smith-Palmer, Truis
AU  - Smith-Palmer T
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Appl Spectrosc
JT  - Applied spectroscopy
JID - 0372406
RN  - 0 (Albumins)
RN  - 0 (Colloids)
RN  - 0 (Phosphatidylcholines)
RN  - 2FP81O2L9Z (Hydroxylamine)
RN  - 3M4G523W1G (Silver)
RN  - 63231-63-0 (RNA)
RN  - IY9XDZ35W2 (Glucose)
RN  - K3Z4F929H6 (Lysine)
SB  - IM
MH  - Albumins/chemistry
MH  - Bacteria/chemistry
MH  - Colloids/*chemistry
MH  - Glucose/chemistry
MH  - Hydrogen-Ion Concentration
MH  - Hydroxylamine/*chemistry
MH  - Lysine/chemistry
MH  - Microscopy, Atomic Force
MH  - Phosphatidylcholines/chemistry
MH  - RNA/chemistry
MH  - Silver/*chemistry
MH  - Spectroscopy, Fourier Transform Infrared
MH  - Spectrum Analysis, Raman/*methods
EDAT- 2009/02/14 09:00
MHDA- 2009/04/08 09:00
CRDT- 2009/02/14 09:00
PHST- 2009/02/14 09:00 [entrez]
PHST- 2009/02/14 09:00 [pubmed]
PHST- 2009/04/08 09:00 [medline]
AID - 10.1366/000370209787391987 [doi]
PST - ppublish
SO  - Appl Spectrosc. 2009 Feb;63(2):214-23. doi: 10.1366/000370209787391987.