PMID- 19215789
OWN - NLM
STAT- MEDLINE
DCOM- 20090220
LR  - 20220317
IS  - 1873-4456 (Electronic)
IS  - 0165-4608 (Linking)
VI  - 189
IP  - 2
DP  - 2009 Mar
TI  - Utility of fluorescence in situ hybridization in the diagnosis of upper urinary 
      tract urothelial carcinoma.
PG  - 93-7
LID - 10.1016/j.cancergencyto.2008.10.015 [doi]
AB  - The purpose of this study was to evaluate the clinical utility of fluorescence in 
      situ hybridization (FISH) assay as a non-invasive method for diagnosing and 
      monitoring urothelial carcinoma (UC) in the upper urinary tract (UUT). Urine 
      specimens from 21 consecutive patients with UUT-UC and 10 healthy controls were 
      analyzed by means of cytology and FISH. For FISH analysis, labeled probes 
      specific for chromosomes 3, 7, and 17 and for the p16 (9p21) gene were used to 
      assess chromosomal abnormalities indicative of malignancy. Sensitivity and 
      specificity of both techniques were determined and compared. The frequency of 
      chromosomal aberrations of malignant cells from UUT was also determined. Overall 
      sensitivity of FISH was significantly higher than that of urine cytology (85.7% 
      vs. 23.8%, p = 0.0009). Specificities for both FISH and cytology were 100% (p = 
      ns). Of 21 patients with UUT-UC, polysomies of chromosome 3, 7 and 17 were 
      observed in 57.1%, 52.4% and 28.6%, respectively, and loss of the p16 gene in 
      47.6%. FISH has a higher sensitivity than cytology and a similar specificity in 
      dectecting UUT-UC. It may be a promising non-invasive tool for the diagnosis and 
      surveillance of UUT-UC.
FAU - Luo, Bin
AU  - Luo B
AD  - Department of Urology, The First Affiliated Hospital of Sun Yet-Sen University, 
      58 Zhongshan 2nd Road, Guangzhou 510080, P.R. China.
FAU - Li, Wen
AU  - Li W
FAU - Deng, Chun-Hua
AU  - Deng CH
FAU - Zheng, Fu-Fu
AU  - Zheng FF
FAU - Sun, Xiang-Zhou
AU  - Sun XZ
FAU - Wang, Dao-Hu
AU  - Wang DH
FAU - Dai, Yu-Ping
AU  - Dai YP
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma/*diagnosis/pathology/urine
MH  - Disease Progression
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - Predictive Value of Tests
MH  - Sensitivity and Specificity
MH  - Urinalysis/*methods
MH  - Urologic Neoplasms/*diagnosis/pathology/urine
MH  - Urothelium/pathology
EDAT- 2009/02/14 09:00
MHDA- 2009/02/21 09:00
CRDT- 2009/02/14 09:00
PHST- 2008/09/16 00:00 [received]
PHST- 2008/10/14 00:00 [accepted]
PHST- 2009/02/14 09:00 [entrez]
PHST- 2009/02/14 09:00 [pubmed]
PHST- 2009/02/21 09:00 [medline]
AID - S0165-4608(08)00627-4 [pii]
AID - 10.1016/j.cancergencyto.2008.10.015 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2009 Mar;189(2):93-7. doi: 
      10.1016/j.cancergencyto.2008.10.015.