PMID- 19218821
OWN - NLM
STAT- MEDLINE
DCOM- 20090721
LR  - 20181201
IS  - 1423-0097 (Electronic)
IS  - 1018-2438 (Linking)
VI  - 149
IP  - 3
DP  - 2009
TI  - Cysteinyl leukotrienes induce monocyte chemoattractant protein-1 in human 
      monocyte/macrophages via mitogen-activated protein kinase and nuclear 
      factor-kappaB pathways.
PG  - 275-82
LID - 10.1159/000199724 [doi]
AB  - BACKGROUND: We have previously demonstrated that cysteinyl leukotriene (CysLT) 
      induced monocyte chemoattractant protein-1 (MCP-1) production in 
      monocytes/macrophages. The intracellular signal transduction pathway of MCP-1 
      production induced by CysLT in human monocytes/macrophages is unclear. METHODS: 
      The activation of mitogen-activated protein kinase (MAPK), including 
      extracellular signal-regulated kinases 1/2 (ERK1/2), c-Jun N-terminal kinase 
      (JNK) and p38 MAPK by phosphorylation, and nuclear factor-kappaB (NF-kappaB) by 
      leukotriene (LT) D(4) and LTC(4) was determined in THP-1 cells, a human monocytic 
      leukemia cell line, and peripheral blood CD14+ monocytes/macrophages. We examined 
      the inhibitory effects of inhibitors of ERK1/2, JNK, p38 MAPK and NF-kappaB and 
      pranlukast as a CysLT1 receptor antagonist on induction of MCP-1 production by 
      LTD(4) and LTC(4). RESULTS: LTD(4) and LTC(4) induced significant 
      phosphorylations of ERK1/2 and JNK, but not p38 MAPK, in THP-1 cells and 
      peripheral blood CD14+ monocytes/macrophages. Pretreatment with the ERK1/2 
      inhibitor PD98059 and JNK inhibitor SP600125 attenuated MCP-1 production by 
      CysLTs. NF-kappaB activation was induced by addition of LTD(4) and LTC(4). 
      Pretreatment with the NF-kappaB inhibitors caffeic acid phenylethyl ester and 
      MG-132 inhibited MCP-1 production by CysLTs. Pranlukast inhibited phosphorylation 
      of ERK1/2 and JNK, NF-kappaB activation, and the MCP-1 production induced by 
      CysLTs. CONCLUSION: CysLTs induce MCP-1 and this induction is mediated by ERK1/2 
      and JNK in MAPK, and NF-kappaB pathways via the CysLT1 receptor, for the most 
      part, in human monocytes/macrophages.
FAU - Hashimoto, Kunio
AU  - Hashimoto K
AD  - Department of Pediatrics, Yamaguchi University Graduate School of Medicine, Ube, 
      Yamaguchi, Japan.
FAU - Ichiyama, Takashi
AU  - Ichiyama T
FAU - Hasegawa, Masanari
AU  - Hasegawa M
FAU - Hasegawa, Shunji
AU  - Hasegawa S
FAU - Matsubara, Tomoyo
AU  - Matsubara T
FAU - Furukawa, Susumu
AU  - Furukawa S
LA  - eng
PT  - Journal Article
DEP - 20090212
PL  - Switzerland
TA  - Int Arch Allergy Immunol
JT  - International archives of allergy and immunology
JID - 9211652
RN  - 0 (Chemokine CCL2)
RN  - 0 (NF-kappa B)
RN  - 0 (Protein Kinase Inhibitors)
RN  - 0 (Receptors, Leukotriene)
RN  - 2CU6TT9V48 (Leukotriene C4)
RN  - 73836-78-9 (Leukotriene D4)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - LRF7RW46ID (leukotriene D4 receptor)
SB  - IM
MH  - Cell Line, Tumor
MH  - Chemokine CCL2/*biosynthesis
MH  - Humans
MH  - Leukotriene C4/*immunology/pharmacology
MH  - Leukotriene D4/*immunology/pharmacology
MH  - Macrophages/drug effects/*immunology
MH  - Mitogen-Activated Protein Kinases/drug effects/*metabolism
MH  - Monocytes/drug effects/immunology
MH  - NF-kappa B/agonists/*metabolism
MH  - Phosphorylation/drug effects
MH  - Protein Kinase Inhibitors/pharmacology
MH  - Receptors, Leukotriene/immunology
MH  - Signal Transduction/drug effects/physiology
EDAT- 2009/02/17 09:00
MHDA- 2009/07/22 09:00
CRDT- 2009/02/17 09:00
PHST- 2008/04/28 00:00 [received]
PHST- 2008/10/07 00:00 [accepted]
PHST- 2009/02/17 09:00 [entrez]
PHST- 2009/02/17 09:00 [pubmed]
PHST- 2009/07/22 09:00 [medline]
AID - 000199724 [pii]
AID - 10.1159/000199724 [doi]
PST - ppublish
SO  - Int Arch Allergy Immunol. 2009;149(3):275-82. doi: 10.1159/000199724. Epub 2009 
      Feb 12.