PMID- 19219701
OWN - NLM
STAT- MEDLINE
DCOM- 20090414
LR  - 20090216
IS  - 1464-5157 (Electronic)
IS  - 0265-6736 (Linking)
VI  - 25
IP  - 1
DP  - 2009 Feb
TI  - Measuring urethral tissue heat injury temperature of healthy male rabbits during 
      interstitial radiofrequency ablation.
PG  - 56-64
LID - 10.1080/02656730802477666 [doi]
AB  - OBJECTIVE: The objective was to evaluate urethral tissue heat injury in a rabbit 
      model. Histopathology analysis was used to determine the minimum temperature 
      required to achieve urethral tissue injury following interstitial radiofrequency 
      ablation (RFA). MATERIALS AND METHODS: A total of 37 healthy rabbits were divided 
      into 6 groups randomly and treated with interstitial RFA in the penial 
      parenchyma. The temperatures of urethra were monitored and controlled to 48 
      degrees , 49 degrees , 50 degrees , 51 degrees 52 degrees and 53 degrees C 
      respectively. The urethral tissue acute heat injury (48 h after heating) was 
      assessed by HE stain, Terminal Deoxylnucleotidyl Transferase Mediated-dUTP Nick 
      End Labeling (TUNEL) stain and quantitative analysis in tissue sections. RESULTS: 
      Histologically, the main feature of acute heat damage was necrosis or vascular 
      congestion or thrombosis of blood vessels of the urethral wall. This occurred 
      only in one out of five cases at 49 degrees C and 50 degrees C heating, but in 
      four out of five cases at 52 degrees and 53 degrees C. The percentage of necrosis 
      was significantly different at tissue temperature of 52 degrees C and above. 
      Quantitative image analyses of TUNEL stain sections demonstrated a significant 
      increase in the positive staining for apoptotic cell at tissue temperatures of 50 
      degrees C and above. It indicated that the TUNEL stain to detect cell death was 
      more sensitive than routine histology. CONCLUSION: The results from this in vivo 
      study indicate that 50 degrees C and 5 min heating of rabbit urethra during 
      interstitial RFA is the minimum temperature for heat injury of the normal rabbit 
      urethra as measured at 48 h after treatment.
FAU - Huang, Ying
AU  - Huang Y
AD  - Department of Ultrasound, Shengjing Hospital of China Medical University, 
      Liaoning Province, China.
FAU - Hu, Bing
AU  - Hu B
FAU - Liu, Da
AU  - Liu D
FAU - Liu, Shou-Jun
AU  - Liu SJ
FAU - Shen, E
AU  - Shen E
FAU - Wu, Rong
AU  - Wu R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Int J Hyperthermia
JT  - International journal of hyperthermia : the official journal of European Society 
      for Hyperthermic Oncology, North American Hyperthermia Group
JID - 8508395
SB  - IM
MH  - Animals
MH  - Apoptosis/physiology
MH  - *Catheter Ablation/adverse effects
MH  - Hot Temperature/*adverse effects
MH  - Humans
MH  - *Hyperthermia, Induced/adverse effects
MH  - In Situ Nick-End Labeling
MH  - Male
MH  - Rabbits
MH  - Random Allocation
MH  - *Temperature
MH  - Urethra/cytology/*injuries/pathology
EDAT- 2009/02/17 09:00
MHDA- 2009/04/15 09:00
CRDT- 2009/02/17 09:00
PHST- 2009/02/17 09:00 [entrez]
PHST- 2009/02/17 09:00 [pubmed]
PHST- 2009/04/15 09:00 [medline]
AID - 908708148 [pii]
AID - 10.1080/02656730802477666 [doi]
PST - ppublish
SO  - Int J Hyperthermia. 2009 Feb;25(1):56-64. doi: 10.1080/02656730802477666.