PMID- 19219750
OWN - NLM
STAT- MEDLINE
DCOM- 20090406
LR  - 20190911
IS  - 1366-5928 (Electronic)
IS  - 0049-8254 (Linking)
VI  - 39
IP  - 1
DP  - 2009 Jan
TI  - Mechanisms of action of two different natural mixtures of persistent organic 
      pollutants (POPs) in ovarian follicles.
PG  - 80-9
LID - 10.1080/00498250802578468 [doi]
AB  - The present work investigated the effects of two different natural mixtures on 
      aryl hydrocarbon receptor (AhR) and oestrogen receptor (ER)beta protein levels, 
      as well as on the activity of cytochrome P450 (CYP) 1A1 and CYP2B. Consequently, 
      the authors observed the effects of these mixtures on gonadotropine-stimulated 
      steroid secretion by ovarian follicles. The natural mixtures that were studied 
      were 'Mjosa' extracted from burbot liver, which contains a high level of PBDEs, 
      and 'Marine mix', extracted from Atlantic cod liver, which contains a high level 
      of polychlorinated biphenyls (PCBs). Follicular cells were exposed in vitro to 
      'Marine mix' and 'Mjosa mix' at doses of 3.6 and 1.4 microg ml(-1), respectively. 
      Media were collected and used for steroid analysis and cell viability assays. 
      Cells were used to estimate aromatase activity (CYP19), AhR and ER protein 
      levels, and CYP1A1 and CYP2B1 activity. Western blot analysis indicated 
      down-regulation of AhR by 'Marine mix' and down-regulation of ERbeta by Mjosa 
      mix. Up-regulation of CYP1A1 expression and activity were seen following 
      treatment with Marine mix, but not Mjosa mix. Increased CYP2B1 activity was noted 
      after treatment with both 'Marine mix' and Mjosa mix. Both mixtures increased 
      luteinizing hormone (LH)-stimulated progesterone and testosterone secretion, 
      follicle-stimulating hormone (FSH)-stimulated oestradiol secretion, and CYP19 
      activity. These results suggest that: (1) 'Marine mix' is a mixed-type CYP 
      inducer; (2) 'Mjosa mix' is an inducer of ERbeta and CYP2B; and (3) both 'Marine 
      mix' and 'Mjosa mix' stimulate aromatase activity as a consequence of oestradiol 
      secretion through activation of CYP19.
FAU - Gregoraszczuk, E L
AU  - Gregoraszczuk EL
AD  - Departament of Physiology and Toxicology of Reproduction, Institute of Zoology, 
      Jagiellonian University, Krakow, Poland. ewa.gregoraszczuk@uj.edu.pl
FAU - Ptak, A
AU  - Ptak A
FAU - Skaare, J U
AU  - Skaare JU
FAU - Mularz, K
AU  - Mularz K
FAU - Chmielowiec, A
AU  - Chmielowiec A
FAU - Wojtowicz, A
AU  - Wojtowicz A
FAU - Ropstad, E
AU  - Ropstad E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Xenobiotica
JT  - Xenobiotica; the fate of foreign compounds in biological systems
JID - 1306665
RN  - 0 (Cell Extracts)
RN  - 0 (Complex Mixtures)
RN  - 0 (Environmental Pollutants)
RN  - 0 (Estrogen Receptor beta)
RN  - 0 (Gonadotropins)
RN  - 0 (Halogenated Diphenyl Ethers)
RN  - 0 (Receptors, Aryl Hydrocarbon)
RN  - 4TI98Z838E (Estradiol)
RN  - 7REL09ZX35 (pentabromodiphenyl ether)
RN  - 9035-51-2 (Cytochrome P-450 Enzyme System)
RN  - DFC2HB4I0K (Polychlorinated Biphenyls)
SB  - IM
MH  - Animals
MH  - Cell Extracts/toxicity
MH  - Cell Survival/physiology
MH  - Cells, Cultured
MH  - Complex Mixtures/chemistry/toxicity
MH  - Cytochrome P-450 Enzyme System/chemistry/metabolism
MH  - Environmental Pollutants/*toxicity
MH  - Enzyme Activation
MH  - Estradiol/metabolism
MH  - Estrogen Receptor beta/*agonists/analysis
MH  - Female
MH  - Gonadotropins/pharmacology
MH  - Halogenated Diphenyl Ethers/analysis/*toxicity
MH  - Ovarian Follicle/*drug effects/metabolism
MH  - Polychlorinated Biphenyls/analysis/*toxicity
MH  - Receptors, Aryl Hydrocarbon/*agonists/chemistry
MH  - Swine
EDAT- 2009/02/17 09:00
MHDA- 2009/04/07 09:00
CRDT- 2009/02/17 09:00
PHST- 2009/02/17 09:00 [entrez]
PHST- 2009/02/17 09:00 [pubmed]
PHST- 2009/04/07 09:00 [medline]
AID - 908728052 [pii]
AID - 10.1080/00498250802578468 [doi]
PST - ppublish
SO  - Xenobiotica. 2009 Jan;39(1):80-9. doi: 10.1080/00498250802578468.