PMID- 19220254
OWN - NLM
STAT- MEDLINE
DCOM- 20090902
LR  - 20211020
IS  - 1464-410X (Electronic)
IS  - 1464-4096 (Print)
IS  - 1464-4096 (Linking)
VI  - 104
IP  - 1
DP  - 2009 Jul
TI  - Superoxide from NADPH oxidase as second messenger for the expression of 
      osteopontin and monocyte chemoattractant protein-1 in renal epithelial cells 
      exposed to calcium oxalate crystals.
PG  - 115-20
LID - 10.1111/j.1464-410X.2009.08374.x [doi]
AB  - OBJECTIVE To test the hypothesis that exposure of a renal epithelial cell line, 
      NRK52E, to calcium oxalate monohydrate crystals (COM) would up-regulate NADPH 
      oxidase subunit p47(phox), enhance superoxide production and increase monocyte 
      chemoattractant protein-1 (MCP-1) and osteopontin mRNA levels. MATERIALS AND 
      METHODS Confluent cultures of NRK52E cells were exposed to COM (66.7 
      microg/cm(2)) with or with no pretreatment with diphenileneiodium chloride (DPI, 
      10 x 10(-6)m) an inhibitor for NADPH oxidase, under serum-free conditions. The 
      conditioned medium was collected and total cellular RNA isolated from the cells, 
      and subjected to enzyme-linked immunosorbent assay and real-time polymerase chain 
      reaction (PCR). Production of reactive oxygen species (ROS) was estimated by 
      dihydroethidium (DHE) staining using a fluorescence microscope. 
      Immunohistochemistry and real-time PCR were used to analyse p47(phox) in NRK52E 
      cells. RESULTS In COM treated NRK52E cells there was enhanced expression of 
      p47(phox) and production of superoxide. COM-induced production of MCP-1 and 
      osteopontin was significantly reduced after treatment with DPI. CONCLUSIONS While 
      the generation of a lot of ROS might play a major role in tissue injury or death, 
      the regulated generation of low concentration of ROS, possibly by NADPH oxidase, 
      may represent a second messenger system for generation of COM-induced MCP-1 and 
      osteopontin production in the renal tubules.
FAU - Umekawa, Tohru
AU  - Umekawa T
AD  - Department of Urology, Kinki University School of Medicine, Osaka, Japan.
FAU - Tsuji, Hidenori
AU  - Tsuji H
FAU - Uemura, Hirotsugu
AU  - Uemura H
FAU - Khan, Saeed R
AU  - Khan SR
LA  - eng
GR  - R01 DK078602/DK/NIDDK NIH HHS/United States
PT  - Journal Article
DEP - 20090210
PL  - England
TA  - BJU Int
JT  - BJU international
JID - 100886721
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
RN  - 106441-73-0 (Osteopontin)
RN  - 11062-77-4 (Superoxides)
RN  - 2612HC57YE (Calcium Oxalate)
RN  - EC 1.6.3.- (NADPH Oxidases)
SB  - IM
MH  - Animals
MH  - Calcium Oxalate/pharmacology
MH  - Cell Line
MH  - Chemokine CCL2/*metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epithelial Cells
MH  - Humans
MH  - Immunohistochemistry
MH  - Kidney Calculi/etiology/*metabolism
MH  - Kidney Tubules/metabolism
MH  - NADPH Oxidases/*metabolism
MH  - Osteopontin/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Second Messenger Systems/*physiology
MH  - Superoxides/*metabolism
MH  - Up-Regulation/physiology
PMC - PMC3711191
MID - NIHMS467640
EDAT- 2009/02/18 09:00
MHDA- 2009/09/03 06:00
PMCR- 2013/07/15
CRDT- 2009/02/18 09:00
PHST- 2009/02/18 09:00 [entrez]
PHST- 2009/02/18 09:00 [pubmed]
PHST- 2009/09/03 06:00 [medline]
PHST- 2013/07/15 00:00 [pmc-release]
AID - BJU8374 [pii]
AID - 10.1111/j.1464-410X.2009.08374.x [doi]
PST - ppublish
SO  - BJU Int. 2009 Jul;104(1):115-20. doi: 10.1111/j.1464-410X.2009.08374.x. Epub 2009 
      Feb 10.