PMID- 19220324
OWN - NLM
STAT- MEDLINE
DCOM- 20090616
LR  - 20211020
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Print)
IS  - 0009-9104 (Linking)
VI  - 156
IP  - 1
DP  - 2009 Apr
TI  - Interaction between prostaglandin D and chemoattractant receptor-homologous 
      molecule expressed on Th2 cells mediates cytokine production by Th2 lymphocytes 
      in response to activated mast cells.
PG  - 126-33
LID - 10.1111/j.1365-2249.2008.03871.x [doi]
AB  - The mechanisms by which immunologically activated mast cells stimulate the 
      production of proinflammatory cytokines by T helper type 2 (Th2) lymphocytes were 
      investigated in a human cell culture system. Supernatants collected from cord 
      blood-derived mast cells after treatment with immunoglobulin E (IgE)/anti-IgE 
      contained an activity that stimulated the production of interleukin (IL)-4, IL-5 
      and IL-13 (both mRNA and protein) by Th2 lymphocytes. This activity was not 
      detected in supernatants from unactivated mast cells and its production was 
      inhibited by treatment of activated mast cells with the cyclo-oxygenase inhibitor 
      diclofenac. The concentration of diclofenac used inhibited completely the 
      production of prostaglandin D(2) (PGD(2)) but did not inhibit the release of 
      histamine or leukotriene C(4). The effect of supernatants from activated mast 
      cells was mimicked by exogenous PGD(2) at concentrations similar to those 
      detected in the cultures of activated mast cells, and addition of exogenous 
      PGD(2) to supernatants from diclofenac-treated mast cells restored their ability 
      to stimulate Th2 cytokine production. The ability of the mast cell supernatants 
      to stimulate production of Th2 cytokines was not affected by addition of 
      diclofenac to the Th2 cells directly, indicating that the production, but not the 
      action, of the factor was sensitive to diclofenac treatment. Inhibition of 
      chemoattractant receptor-homologous molecule expressed on Th2 cells (CRTH2) 
      abolished the effect of the mast cell supernatants on Th2 cytokine production. 
      These data indicate that mast cells have the ability to stimulate Th2 cells to 
      elaborate cytokines independently of T cell receptor activation or co-stimulation 
      and this response is mediated by PGD(2) acting upon CRTH2 expressed by Th2 cells.
FAU - Xue, L
AU  - Xue L
AD  - Oxagen Ltd, Abingdon, Oxon, UK. xue@oxagen.co.uk
FAU - Barrow, A
AU  - Barrow A
FAU - Pettipher, R
AU  - Pettipher R
LA  - eng
PT  - Journal Article
DEP - 20090211
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Cytokines)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Receptors, Prostaglandin)
RN  - RXY07S6CZ2 (Prostaglandin D2)
RN  - XZF106QU24 (prostaglandin D2 receptor)
SB  - IM
MH  - Cells, Cultured
MH  - Culture Media, Conditioned
MH  - Cytokines/*biosynthesis/genetics
MH  - Gene Expression Regulation/immunology
MH  - Humans
MH  - Mast Cells/*immunology
MH  - Prostaglandin D2/immunology/*metabolism
MH  - RNA, Messenger/analysis
MH  - Receptors, Immunologic/immunology/*metabolism
MH  - Receptors, Prostaglandin/immunology/*metabolism
MH  - Th2 Cells/*immunology
PMC - PMC2673750
EDAT- 2009/02/18 09:00
MHDA- 2009/06/17 09:00
PMCR- 2010/04/01
CRDT- 2009/02/18 09:00
PHST- 2009/02/18 09:00 [entrez]
PHST- 2009/02/18 09:00 [pubmed]
PHST- 2009/06/17 09:00 [medline]
PHST- 2010/04/01 00:00 [pmc-release]
AID - CEI3871 [pii]
AID - 10.1111/j.1365-2249.2008.03871.x [doi]
PST - ppublish
SO  - Clin Exp Immunol. 2009 Apr;156(1):126-33. doi: 10.1111/j.1365-2249.2008.03871.x. 
      Epub 2009 Feb 11.