PMID- 19220581
OWN - NLM
STAT- MEDLINE
DCOM- 20111223
LR  - 20211203
IS  - 1582-4934 (Electronic)
IS  - 1582-1838 (Print)
IS  - 1582-1838 (Linking)
VI  - 13
IP  - 9B
DP  - 2009 Sep
TI  - Extracellular calcium increases CXCR4 expression on bone marrow-derived cells and 
      enhances pro-angiogenesis therapy.
PG  - 3764-73
LID - 10.1111/j.1582-4934.2009.00691.x [doi]
AB  - Cell surface receptors play major roles in the mobilization and homing of 
      progenitor cells from the bone marrow to peripheral tissues. CXCR4 is an 
      important receptor that regulates homing of leucocytes and endothelial 
      progenitors in response to the chemokine stromal cell-derived factor-1 (SDF-1). 
      Ionic calcium is also known to regulate chemotaxis of selective bone marrow cells 
      (BMCs) through the calcium-sensing receptor, CaR. Here we show that calcium 
      regulates CXCR4 expression and BMC responses to SDF-1. CaCl(2) treatment of BMC 
      induced a time- and dose-dependent increase in both the transcription and cell 
      surface expression of CXCR4. BMC subpopulations expressing VEGFR2(+), CD34(+) and 
      cKit(+)/Sca-1(+) were especially sensitive to calcium. The effects were blocked 
      by calcium influx inhibitors, anti-CaR antibody and the protein synthesis 
      inhibitor cycloheximide, but not by the CXCR4 antagonist AMD3100. Calcium 
      treatment also enhanced SDF-1-mediated CXCR4 internalization. These changes were 
      reflected in significantly improved chemotaxis by SDF-1, which was abolished by 
      AMD3100 and by antibody against CXCR4. Calcium pre-treatment improved homing of 
      CD34(+) BMCs to ischemic muscle in vivo, and enhanced revascularization in 
      ischemic mouse hindlimbs. Our results identify calcium as a positive regulator of 
      CXCR4 expression that promotes stem cell mobilization, homing and therapy.
FAU - Wu, Quiling
AU  - Wu Q
AD  - Vascular Biology Institute, University of Miami, Miller School of Medicine, 
      Miami, FL 33136, USA.
FAU - Shao, Hongwei
AU  - Shao H
FAU - Darwin, Eton D
AU  - Darwin ED
FAU - Li, Jiahui
AU  - Li J
FAU - Li, Jie
AU  - Li J
FAU - Yang, Bing
AU  - Yang B
FAU - Webster, Keith A
AU  - Webster KA
FAU - Yu, Hong
AU  - Yu H
LA  - eng
GR  - R01 HL072924/HL/NHLBI NIH HHS/United States
GR  - R01 HL072924-04/HL/NHLBI NIH HHS/United States
GR  - R01 HL092499/HL/NHLBI NIH HHS/United States
GR  - R01 HL092499-01A1/HL/NHLBI NIH HHS/United States
PT  - Journal Article
DEP - 20090209
PL  - England
TA  - J Cell Mol Med
JT  - Journal of cellular and molecular medicine
JID - 101083777
RN  - 0 (Benzylamines)
RN  - 0 (Cyclams)
RN  - 0 (Heterocyclic Compounds)
RN  - 0 (Receptors, CXCR4)
RN  - 0 (Receptors, Calcium-Sensing)
RN  - 98600C0908 (Cycloheximide)
RN  - S915P5499N (plerixafor)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Benzylamines
MH  - Bone Marrow Cells/*metabolism
MH  - Calcium/*metabolism
MH  - Chemotaxis
MH  - Cyclams
MH  - Cycloheximide/pharmacology
MH  - Dose-Response Relationship, Drug
MH  - *Gene Expression Regulation
MH  - Hematopoietic Stem Cell Mobilization
MH  - Heterocyclic Compounds/pharmacology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - *Neovascularization, Physiologic
MH  - Receptors, CXCR4/*biosynthesis
MH  - Receptors, Calcium-Sensing/metabolism
MH  - Stem Cells/cytology
PMC - PMC3124762
MID - NIHMS305539
EDAT- 2009/02/18 09:00
MHDA- 2011/12/24 06:00
PMCR- 2009/09/01
CRDT- 2009/02/18 09:00
PHST- 2009/02/18 09:00 [entrez]
PHST- 2009/02/18 09:00 [pubmed]
PHST- 2011/12/24 06:00 [medline]
PHST- 2009/09/01 00:00 [pmc-release]
AID - JCMM691 [pii]
AID - 10.1111/j.1582-4934.2009.00691.x [doi]
PST - ppublish
SO  - J Cell Mol Med. 2009 Sep;13(9B):3764-73. doi: 10.1111/j.1582-4934.2009.00691.x. 
      Epub 2009 Feb 9.