PMID- 19220953
OWN - NLM
STAT- MEDLINE
DCOM- 20100506
LR  - 20090217
IS  - 1003-0603 (Print)
IS  - 1003-0603 (Linking)
VI  - 21
IP  - 2
DP  - 2009 Feb
TI  - [The comparative proteomic analysis of serum cytokine expression in acute lung 
      injury patients in peri-operative stage of liver transplantation].
PG  - 74-8
AB  - OBJECTIVE: To investigate the characteristics of serum cytokine expression in 
      acute lung injury (ALI) patients in peri-operative stage of liver transplantation 
      with the aim of setting the basis for screening the early markers and treatment 
      targets of ALI. METHODS: Four male patients with ALI occurring in peri-operative 
      stage of liver transplantation for hepatitis B liver cirrhosis, with no lung, 
      renal, or brain abnormality, without difference in clinical findings (urine 
      volume, blood loss, ascites, amount of blood transfusion, operation time, 
      anhepatic time, the use of vaso-active drugs, diuretics and condition of 
      circulation) were included for study. Blood was taken after anesthesia, 3 hours 
      and 24 hours after new liver. RayBio human antibody array was used to analyze the 
      cytokine expression. RESULTS: Compared with healthy people, in the patients with 
      ALI in peri-operative stage of liver transplantation, upregulation of some 
      cytokines appeared as early as after anesthesia, including interleukins (IL-3, 
      IL-6, IL-12 p40, IL-12 p70), monocyte chemoattractant protein-2 (MCP-2), 
      macrophage-colony stimulating factor (M-CSF), monokine induced by 
      interferon-gamma (MIG), macro-phage inflammatory protein-1 alpha (MIP-1 alpha), 
      soluble tumor necrosis factor receptor I (sTNFR I), especially sTNFR II which 
      showed even stronger expression, while normal T cells expression and secretory 
      factor (RANTES) and platelet-derived growth factor-BB (PDGF-BB) showed 
      downregulation in expression. Some more cytokines showed upregulation in 
      expression at neohepatic 3 hours, especially IL-12 p70, sTNFR I, sTNFR II showed 
      upregulation, while RANTES, PDGF-BB and IL-1 alpha showed downregulation in 
      expression. The number of cytokines showing upregulation was significantly 
      increased at neohepatic 24 hours. Compared with those at neohepatic 3 hours, 
      eotaxin, IL-1 alpha, IL-1 beta, IL-4, IL-15, MCP-2 showed significantly higher 
      upregulation at neohepatic 24 hours, and among them IL-3 and IL-6 especially IL-2 
      showed even more upregulation in expression. CONCLUSION: There are changes in 
      expression of different kinds of cytokines in various extent before operation, at 
      neohepatic 3 hours and neohepatic 24 hours. Some of them may be considered as 
      important early markers and treatment targets. Further researches with large 
      samples would be necessary to elucidate the clinical implication.
FAU - Guo, Na
AU  - Guo N
AD  - The Anesthesia Department of the Third Affiliated Hospital of Sun Yat-sen 
      University, Guangzhou 510630, Guangdong, China.
FAU - Hei, Zi-qing
AU  - Hei ZQ
FAU - Pang, Hong-yu
AU  - Pang HY
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Wei Zhong Bing Ji Jiu Yi Xue
JT  - Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo 
      weizhongbing jijiuyixue
JID - 9887521
RN  - 0 (Chemokine CCL8)
RN  - 0 (Cytokines)
RN  - 0 (Interleukins)
RN  - 81627-83-0 (Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Acute Lung Injury/*blood
MH  - Adult
MH  - Chemokine CCL8/blood
MH  - Cytokines/*blood
MH  - Humans
MH  - Interleukins/blood
MH  - Intraoperative Period
MH  - *Liver Transplantation
MH  - Macrophage Colony-Stimulating Factor/blood
MH  - Male
MH  - Middle Aged
MH  - *Proteomics
EDAT- 2009/02/18 09:00
MHDA- 2010/05/07 06:00
CRDT- 2009/02/18 09:00
PHST- 2009/02/18 09:00 [entrez]
PHST- 2009/02/18 09:00 [pubmed]
PHST- 2010/05/07 06:00 [medline]
PST - ppublish
SO  - Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2009 Feb;21(2):74-8.