PMID- 1922103
OWN - NLM
STAT- MEDLINE
DCOM- 19911113
LR  - 20190824
IS  - 0161-5890 (Print)
IS  - 0161-5890 (Linking)
VI  - 28
IP  - 10
DP  - 1991 Oct
TI  - Preferential binding and aggregation of rabbit C-reactive protein with 
      arginine-rich proteins.
PG  - 1113-20
AB  - Human and rabbit C-reactive proteins (CRP) are similar in mol. wt, structure and 
      amino acid sequence. In addition to the characteristic phosphoryline (PC)-binding 
      specificity, both CRP molecules bind arginine- and lysine-rich proteins. The 
      human CRP-cationic protein reactivity has been reported to be inhibited by 
      calcium and promoted by PC in the presence of calcium. The present study compares 
      binding and precipitation reactions of rabbit CRP (raCRP) with arginine- and 
      lysine-rich proteins, and demonstrates the differential modulation of these 
      interactions by calcium and acidic pH. Rabbit CRP shows preferential binding and 
      precipitation reactivities with arginine-rich cationic molecules. Binding of 
      raCRP to poly-L-arginine (PLA) and arginine-rich histone (ARH) occurs at pH 6.0, 
      in the presence of calcium and is inhibitable by phosphorylcholine (PC) 
      suggesting an interaction at or near the calcium-modulated PC binding site. The 
      in vitro precipitation of raCRP and arginine-rich cationic molecules is 
      significantly inhibited at pH 6.0, by the non-precipitating lysine-rich ligand 
      PLL, and by physiological levels of calcium, and may reflect the participation of 
      distinct "self-aggregation" sites on CRP in the precipitation response. The 
      significance of the preferential arginine reactivity of raCRP to in vivo 
      functions as a scavenger of chromatin during cell death and/or as a modulator of 
      lipoprotein metabolism during the acute phase response is discussed.
FAU - Dougherty, T J
AU  - Dougherty TJ
AD  - Department of Biology, Purdue University-Calumet, Hammond, IN 46323.
FAU - Gewurz, H
AU  - Gewurz H
FAU - Siegel, J N
AU  - Siegel JN
LA  - eng
PT  - Journal Article
PL  - England
TA  - Mol Immunol
JT  - Molecular immunology
JID - 7905289
RN  - 0 (Cations)
RN  - 0 (Chromatin)
RN  - 0 (Ligands)
RN  - 0 (Peptides)
RN  - 0 (Proteins)
RN  - 25104-18-1 (Polylysine)
RN  - 25212-18-4 (polyarginine)
RN  - 9007-41-4 (C-Reactive Protein)
RN  - 94ZLA3W45F (Arginine)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Arginine/*chemistry
MH  - C-Reactive Protein/*metabolism
MH  - Calcium/metabolism
MH  - Cations
MH  - Chemical Precipitation
MH  - Chromatin/metabolism
MH  - Hydrogen-Ion Concentration
MH  - In Vitro Techniques
MH  - Ligands
MH  - Peptides/metabolism
MH  - Polylysine/metabolism
MH  - Protein Binding
MH  - Proteins/chemistry/*metabolism
MH  - Rabbits
EDAT- 1991/10/01 00:00
MHDA- 1991/10/01 00:01
CRDT- 1991/10/01 00:00
PHST- 1991/10/01 00:00 [pubmed]
PHST- 1991/10/01 00:01 [medline]
PHST- 1991/10/01 00:00 [entrez]
AID - 10.1016/0161-5890(91)90026-g [doi]
PST - ppublish
SO  - Mol Immunol. 1991 Oct;28(10):1113-20. doi: 10.1016/0161-5890(91)90026-g.