PMID- 19223011
OWN - NLM
STAT- MEDLINE
DCOM- 20090817
LR  - 20211020
IS  - 1873-7544 (Electronic)
IS  - 0306-4522 (Print)
IS  - 0306-4522 (Linking)
VI  - 160
IP  - 1
DP  - 2009 Apr 21
TI  - Dose-dependent effects of post-training estradiol plus progesterone treatment on 
      object memory consolidation and hippocampal extracellular signal-regulated kinase 
      activation in young ovariectomized mice.
PG  - 6-12
LID - 10.1016/j.neuroscience.2009.02.024 [doi]
AB  - Previous work from our laboratory has shown that the ability of estradiol to 
      enhance object memory consolidation in young ovariectomized mice is dependent on 
      dorsal hippocampal activation of the extracellular signal-regulated 
      kinase/mitogen-activated protein kinase (ERK/MAPK) signaling pathway [Fernandez 
      SM, Lewis MC, Pechenino AS, Harburger LL, Orr PT, Gresack JE, Schafe GE, Frick KM 
      (2008) Estradiol-induced enhancement of object memory consolidation involves 
      hippocampal extracellular signal-regulated kinase activation and membrane-bound 
      estrogen receptors. J Neurosci 28:8660-8667]. However, it is unclear if estradiol 
      modulates memory or ERK activation similarly in the presence of progesterone. 
      Therefore, the present study investigated effects of combined estradiol and 
      progesterone treatment on object memory consolidation and dorsal hippocampal ERK 
      activation in young ovariectomized C57BL/6 mice. Object memory was tested in a 
      novel object recognition task. Immediately after training, mice received 
      intraperiotoneal (i.p.) injections of vehicle, 17beta-estradiol (E(2); 0.2 
      mg/kg), or E(2) plus 5, 10, or 20 mg/kg progesterone (P). Forty-eight hours 
      later, mice receiving E(2) alone or E(2) plus 10 or 20 mg/kg P exhibited 
      significantly enhanced memory for the novel object relative to chance, whereas 
      those receiving vehicle or E(2) plus 5 mg/kg P spent no more time than chance 
      with the novel object. Two weeks later, ERK phosphorylation was measured in the 
      dorsal hippocampus 1 h after i.p. injection of vehicle, E(2), or E(2) plus P. 
      Consistent with our previous work [Fernandez SM, Lewis MC, Pechenino AS, 
      Harburger LL, Orr PT, Gresack JE, Schafe GE, Frick KM (2008) Estradiol-induced 
      enhancement of object memory consolidation involves hippocampal extracellular 
      signal-regulated kinase activation and membrane-bound estrogen receptors. J 
      Neurosci 28:8660-8667], E(2) alone significantly increased phospho-p42 ERK 
      protein levels in the dorsal hippocampus relative to vehicle controls. In 
      contrast, no combination of E(2) and P affected dorsal hippocampal phospho-ERK 
      levels. These data indicate that, unlike E(2) alone, the beneficial effects of 
      combined E(2) plus P treatment on memory are not associated with ERK activation 
      in the dorsal hippocampus 1 h after treatment, and suggest that E(2) alone and 
      combined E(2) plus P may influence ERK activation in different time frames or 
      enhance memory through different mechanisms.
FAU - Harburger, L L
AU  - Harburger LL
AD  - Department of Psychology, Stern College for Women, Yeshiva University, New York, 
      NY 10016, USA.
FAU - Saadi, A
AU  - Saadi A
FAU - Frick, K M
AU  - Frick KM
LA  - eng
GR  - R01 AG022525-02/AG/NIA NIH HHS/United States
GR  - R01 AG022525-03/AG/NIA NIH HHS/United States
GR  - R01 AG022525/AG/NIA NIH HHS/United States
GR  - R01 AG022525-04/AG/NIA NIH HHS/United States
GR  - R01 AG022525-01A2/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20090214
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Estrogens)
RN  - 0 (Progestins)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 4TI98Z838E (Estradiol)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Dose-Response Relationship, Drug
MH  - Enzyme Activation/drug effects
MH  - Estradiol/*administration & dosage
MH  - Estrogens/*administration & dosage
MH  - Extracellular Signal-Regulated MAP Kinases/*metabolism
MH  - Female
MH  - Hippocampus/drug effects/enzymology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mitogen-Activated Protein Kinase 1/metabolism
MH  - Mitogen-Activated Protein Kinase 3/metabolism
MH  - Ovariectomy
MH  - Phosphorylation/drug effects
MH  - Progesterone/*administration & dosage
MH  - Progestins/*administration & dosage
MH  - Recognition, Psychology/*drug effects
PMC - PMC2668744
MID - NIHMS95881
EDAT- 2009/02/19 09:00
MHDA- 2009/08/18 09:00
PMCR- 2010/04/21
CRDT- 2009/02/19 09:00
PHST- 2008/11/05 00:00 [received]
PHST- 2009/02/11 00:00 [revised]
PHST- 2009/02/12 00:00 [accepted]
PHST- 2009/02/19 09:00 [entrez]
PHST- 2009/02/19 09:00 [pubmed]
PHST- 2009/08/18 09:00 [medline]
PHST- 2010/04/21 00:00 [pmc-release]
AID - S0306-4522(09)00231-0 [pii]
AID - 10.1016/j.neuroscience.2009.02.024 [doi]
PST - ppublish
SO  - Neuroscience. 2009 Apr 21;160(1):6-12. doi: 10.1016/j.neuroscience.2009.02.024. 
      Epub 2009 Feb 14.