PMID- 19224567
OWN - NLM
STAT- MEDLINE
DCOM- 20090610
LR  - 20240312
IS  - 1932-8451 (Print)
IS  - 1932-846X (Electronic)
IS  - 1932-8451 (Linking)
VI  - 69
IP  - 5
DP  - 2009 Apr
TI  - Experience-dependent regulation of TrkB isoforms in rodent visual cortex.
PG  - 267-78
LID - 10.1002/dneu.20701 [doi]
AB  - Within primary visual cortex (V1), brain-derived neurotrophic factor (BDNF) 
      signaling through its high-affinity receptor TrkB is important for normal 
      development and experience-dependent plasticity. TrkB is expressed in several 
      alternatively spliced isoforms, including full-length TrkB (TrkB.FL), and several 
      truncated isoforms (TrkB.T1, TrkB.T2, and TrkB.T4) that lack the intracellular 
      tyrosine kinase domain. These isoforms are important components of BDNF 
      signaling, yet little is known about the developmental or experience-dependent 
      regulation of their expression. Using immunohistochemistry, we found TrkB.FL and 
      TrkB.T1 expressed in interneurons and pyramidal neurons within V1, but not in 
      cortical astrocytes. We used real-time PCR to quantify the changes in mRNA 
      expression of BDNF, the four TrkB isoforms, and the low-affinity receptor P75NTR 
      during normal development, and in response to visual deprivation at two different 
      ages. BDNF expression increased between postnatal days 10 (P10) and P30, and was 
      rapidly down-regulated by 3 days of visual deprivation during both the 
      pre-critical period (P14-P17) and the critical period (P18-P21). Over the same 
      developmental period, expression of each TrkB isoform was regulated 
      independently; TrkB.T1 increased, TrkB.FL and TrkB.T2 decreased, and TrkB.T4 
      showed transient changes. Neither brief visual deprivation nor prolonged 
      dark-rearing induced changes in either TrkB.FL or TrkB.T1 expression. However, 
      TrkB.T4 expression was reduced by brief visual deprivation, whereas TrkB.T4, 
      TrkB.T2 and P75(NTR) were up-regulated by prolonged dark-rearing into the 
      critical period. Our data indicate that TrkB isoform expression can be 
      selectively regulated by visual experience, and may contribute to 
      experience-dependent cortical plasticity.
CI  - Copyright 2009 Wiley Periodicals, Inc. Develop Neurobiol 2009.
FAU - Bracken, Bethany K
AU  - Bracken BK
AD  - Department of Biology, Brandeis University, Waltham, Massachusetts 02454, USA.
FAU - Turrigiano, Gina G
AU  - Turrigiano GG
LA  - eng
GR  - R01 EY014429/EY/NEI NIH HHS/United States
GR  - R01 EY014439/EY/NEI NIH HHS/United States
GR  - R01 EY014439-05/EY/NEI NIH HHS/United States
GR  - EY 014429/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Dev Neurobiol
JT  - Developmental neurobiology
JID - 101300215
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Parvalbumins)
RN  - 0 (Protein Isoforms)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Growth Factor)
RN  - 0 (Receptors, Nerve Growth Factor)
RN  - 136958-07-1 (Ngfr protein, rat)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Age Factors
MH  - Analysis of Variance
MH  - Animals
MH  - Animals, Newborn
MH  - Brain-Derived Neurotrophic Factor/genetics/metabolism
MH  - Dark Adaptation/physiology
MH  - Functional Laterality
MH  - Gene Expression Regulation, Developmental/*physiology
MH  - Nerve Tissue Proteins/metabolism
MH  - Neurons/classification/metabolism
MH  - Parvalbumins/metabolism
MH  - Protein Isoforms/genetics/metabolism
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Long-Evans
MH  - Receptor, trkB/genetics/*metabolism
MH  - Receptors, Growth Factor
MH  - Receptors, Nerve Growth Factor/genetics/metabolism
MH  - Sensory Deprivation/*physiology
MH  - Vision, Binocular/physiology
MH  - Vision, Monocular/physiology
MH  - Visual Cortex/cytology/*metabolism
PMC - PMC2834411
MID - NIHMS178211
EDAT- 2009/02/19 09:00
MHDA- 2009/06/11 09:00
PMCR- 2010/04/01
CRDT- 2009/02/19 09:00
PHST- 2009/02/19 09:00 [entrez]
PHST- 2009/02/19 09:00 [pubmed]
PHST- 2009/06/11 09:00 [medline]
PHST- 2010/04/01 00:00 [pmc-release]
AID - 10.1002/dneu.20701 [doi]
PST - ppublish
SO  - Dev Neurobiol. 2009 Apr;69(5):267-78. doi: 10.1002/dneu.20701.