PMID- 19225407
OWN - NLM
STAT- MEDLINE
DCOM- 20090430
LR  - 20090227
IS  - 0022-3069 (Print)
IS  - 0022-3069 (Linking)
VI  - 68
IP  - 3
DP  - 2009 Mar
TI  - Dendritic cell adhesion to cerebral endothelium: role of endothelial cell 
      adhesion molecules and their ligands.
PG  - 300-13
LID - 10.1097/NEN.0b013e31819a8dd1 [doi]
AB  - Dendritic cells (DCs) have been increasingly implicated in the pathogenesis of 
      neuroinflammation, and there is evidence that they are recruited to the brain 
      across the blood-brain barrier. The molecular mechanisms mediating DC trafficking 
      to the central nervous system are poorly understood. This study used an in vitro 
      model of the human blood-brain barrier and monocyte-derived DCs to investigate 
      the role of endothelial cell (EC) adhesion molecules and their ligands in the 
      adhesion of immature and mature DCs to cerebral microvascular ECs. Adhesion of 
      DCs to resting brain ECs was minimal, but activation of ECs with tumor necrosis 
      factor significantly upregulated adhesion. Immature DCs adhered to activated ECs 
      more avidly than mature DCs; this correlated with differences in the expression 
      of adhesion molecule ligands between the mature and immature DCs. Blocking 
      studies indicated that adhesion to cytokine-activated blood-brain barrier 
      endothelium is mediated by intercellular adhesion molecule (ICAM)-1, ICAM-2, 
      platelet-EC adhesion molecule (PECAM)-1, vascular cell adhesion molecule 1, CD18, 
      and DC-specific ICAM-3-grabbing nonintegrin (DC-SIGN) for immature DCs and 
      ICAM-1, CD18, DC-SIGN, and PECAM-1 for mature DCs. These results suggest that DC 
      adhesion to cerebral ECs depends on the maturation state of DCs and the 
      activation state of the endothelium, and that it is regulated by specific 
      receptor-ligand interactions. This study thus further highlights the active role 
      of human brain microvascular ECs in neuroinflammation.
FAU - Arjmandi, Azadeh
AU  - Arjmandi A
AD  - Department of Pathology and Laboratory Medicine, Division of Neuropathology, 
      Vancouver General Hospital and The University of British Columbia, Vancouver, BC, 
      Canada.
FAU - Liu, Kenneth
AU  - Liu K
FAU - Dorovini-Zis, Katerina
AU  - Dorovini-Zis K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Neuropathol Exp Neurol
JT  - Journal of neuropathology and experimental neurology
JID - 2985192R
RN  - 0 (Cell Adhesion Molecules)
SB  - IM
MH  - Brain/blood supply/*immunology/metabolism
MH  - Cell Adhesion/immunology
MH  - Cell Adhesion Molecules/*immunology/metabolism
MH  - Cell Differentiation/immunology
MH  - Chemotaxis, Leukocyte/*immunology
MH  - Dendritic Cells/cytology/*immunology/metabolism
MH  - Endothelium, Vascular/*immunology/metabolism
MH  - Flow Cytometry
MH  - Humans
MH  - Inflammation/*immunology
EDAT- 2009/02/20 09:00
MHDA- 2009/05/01 09:00
CRDT- 2009/02/20 09:00
PHST- 2009/02/20 09:00 [entrez]
PHST- 2009/02/20 09:00 [pubmed]
PHST- 2009/05/01 09:00 [medline]
AID - 10.1097/NEN.0b013e31819a8dd1 [doi]
PST - ppublish
SO  - J Neuropathol Exp Neurol. 2009 Mar;68(3):300-13. doi: 
      10.1097/NEN.0b013e31819a8dd1.