PMID- 19225715
OWN - NLM
STAT- MEDLINE
DCOM- 20090318
LR  - 20090305
IS  - 1432-8798 (Electronic)
IS  - 0304-8608 (Linking)
VI  - 154
IP  - 3
DP  - 2009
TI  - Role of glyceraldehyde-3-phosphate dehydrogenase binding to hepatitis B virus 
      posttranscriptional regulatory element in regulating expression of HBV surface 
      antigen.
PG  - 519-24
LID - 10.1007/s00705-009-0326-8 [doi]
AB  - The hepatitis B virus (HBV) posttranscriptional regulatory element (HPRE) has 
      been demonstrated to facilitate the cytoplasmic localization of unspliced 
      transcripts. One cellular protein, glyceraldehyde-3-phosphate dehydrogenase 
      (GAPDH), is known to combine with this element. However, its function on HPRE 
      remains unclear. Here, we show that recombinant GAPDH protein binds to HPRE RNA 
      in vitro in streptavidin pull-down assays. Functional analysis demonstrated that 
      GAPDH inhibited HPRE function in a pDM138-HPRE chloramphenicol acetyltransferase 
      reporter assay system. Overexpression of GAPDH depressed the expression of HBs 
      antigen, as detected both in cells transiently expressing HBs-HPRE and in 
      HepG2.2.15 cells. These data indicate that GAPDH may be involved in the 
      posttranscriptional regulation of HBV, which is critical for the life cycle of 
      HBV.
FAU - Li, Yi
AU  - Li Y
AD  - Key Laboratory of Molecular Infectious Diseases, Ministry of Education, Chongqing 
      Medical University, 1 Yi Xue Yuan Road, 400016, Chongqing, China.
FAU - Huang, Tingting
AU  - Huang T
FAU - Zhang, Xiaohua
AU  - Zhang X
FAU - Wan, Tao
AU  - Wan T
FAU - Hu, Jieli
AU  - Hu J
FAU - Huang, Ailong
AU  - Huang A
FAU - Tang, Hua
AU  - Tang H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090219
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (Hepatitis B Surface Antigens)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Viral)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
RN  - EC 2.3.1.28 (Chloramphenicol O-Acetyltransferase)
SB  - IM
MH  - Cell Line
MH  - Chloramphenicol O-Acetyltransferase/genetics/metabolism
MH  - Genes, Reporter
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/*metabolism
MH  - Hepatitis B Surface Antigens/*biosynthesis
MH  - Hepatitis B virus/*physiology
MH  - Humans
MH  - Protein Binding
MH  - RNA, Messenger/*metabolism
MH  - RNA, Viral/*metabolism
EDAT- 2009/02/20 09:00
MHDA- 2009/03/19 09:00
CRDT- 2009/02/20 09:00
PHST- 2008/08/16 00:00 [received]
PHST- 2009/01/29 00:00 [accepted]
PHST- 2009/02/20 09:00 [entrez]
PHST- 2009/02/20 09:00 [pubmed]
PHST- 2009/03/19 09:00 [medline]
AID - 10.1007/s00705-009-0326-8 [doi]
PST - ppublish
SO  - Arch Virol. 2009;154(3):519-24. doi: 10.1007/s00705-009-0326-8. Epub 2009 Feb 19.