PMID- 19228761
OWN - NLM
STAT- MEDLINE
DCOM- 20090825
LR  - 20220409
IS  - 1460-2350 (Electronic)
IS  - 0268-1161 (Linking)
VI  - 24
IP  - 6
DP  - 2009 Jun
TI  - Endometrial secretion analysis identifies a cytokine profile predictive of 
      pregnancy in IVF.
PG  - 1427-35
LID - 10.1093/humrep/dep011 [doi]
AB  - BACKGROUND: The study of human endometrial-embryonic interactions is complicated 
      by the disruptive impact of endometrial sample collection on the process of 
      implantation itself. Endometrial secretion analysis is a novel technique, 
      non-disruptive to implantation. The primary aim of this prospective cohort study 
      was to explore whether a cytokine profile predictive of implantation and clinical 
      pregnancy can be identified in endometrial secretions aspirated immediately prior 
      to embryo transfer following IVF. METHODS: Endometrial secretions, aspirated 
      immediately prior to embryo transfer from 210 women undergoing IVF, were analyzed 
      using a multiplex immunoassay for 17 soluble regulators of implantation, namely 
      interleukin (IL)-1beta, IL-5, IL-6, IL-10, IL-12, IL-15, IL-17, IL-18, tumor 
      necrosis factor (TNF)-alpha, interferon (IFN)-gamma, macrophage migration 
      inhibitory factor, eotaxin, IFN-gamma-inducible 10 kDa protein (IP-10), monocyte 
      chemo-attractant protein-1 (MCP-1), Dickkopf homolog 1, heparin-binding epidermal 
      growth factor and vascular endothelial growth factor (VEGF). In order to detect 
      implantation, daily urine samples were collected after embryo transfer, and human 
      Chorionic Gonadotropin (hCG) concentrations were analyzed by an immunoassay. 
      RESULTS: Multivariable logistic regression analysis revealed significant 
      associations (negative and positive association, respectively) between MCP-1 (P = 
      0.005) and IP-10 (P = 0.037) levels and implantation, and between IL-1beta (P = 
      0.047) and TNF-alpha (P = 0.023) levels and clinical pregnancy. The predictive 
      value for pregnancy of IL-1beta and TNF-alpha was observed to be equivalent and 
      additive to that of embryo quality. CONCLUSIONS: Endometrial secretion cytokine 
      profiling offers a novel, non-disruptive approach to study the role of the 
      endometrium in human embryo implantation and identifies a profile which appears 
      to be conducive to clinical pregnancy. CLINICAL TRIAL REGISTRATION: 
      www.clinicaltrials.gov (nCT00264992).
FAU - Boomsma, C M
AU  - Boomsma CM
AD  - Department of Reproductive Medicine and Gynecology, University Medical Center 
      Utrecht, F05.126, PO Box 85500, 3508 GA, Utrecht, The Netherlands. 
      c.m.boomsma@umcutrecht.nl
FAU - Kavelaars, A
AU  - Kavelaars A
FAU - Eijkemans, M J C
AU  - Eijkemans MJ
FAU - Lentjes, E G
AU  - Lentjes EG
FAU - Fauser, B C J M
AU  - Fauser BC
FAU - Heijnen, C J
AU  - Heijnen CJ
FAU - Macklon, N S
AU  - Macklon NS
LA  - eng
SI  - ClinicalTrials.gov/NCT00264992
PT  - Clinical Trial
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090219
PL  - England
TA  - Hum Reprod
JT  - Human reproduction (Oxford, England)
JID - 8701199
RN  - 0 (Biomarkers)
RN  - 0 (Cytokines)
SB  - IM
MH  - Adult
MH  - Area Under Curve
MH  - Biomarkers/metabolism
MH  - Cytokines/*metabolism
MH  - Embryo Implantation/immunology
MH  - Embryo Transfer
MH  - Endometrium/*immunology/*metabolism
MH  - Female
MH  - Fertilization in Vitro/*methods
MH  - Humans
MH  - Immunoassay/*methods
MH  - Logistic Models
MH  - Multivariate Analysis
MH  - Predictive Value of Tests
MH  - Pregnancy
MH  - Pregnancy Outcome
MH  - Prospective Studies
EDAT- 2009/02/21 09:00
MHDA- 2009/08/26 09:00
CRDT- 2009/02/21 09:00
PHST- 2009/02/21 09:00 [entrez]
PHST- 2009/02/21 09:00 [pubmed]
PHST- 2009/08/26 09:00 [medline]
AID - dep011 [pii]
AID - 10.1093/humrep/dep011 [doi]
PST - ppublish
SO  - Hum Reprod. 2009 Jun;24(6):1427-35. doi: 10.1093/humrep/dep011. Epub 2009 Feb 19.