PMID- 19228887
OWN - NLM
STAT- MEDLINE
DCOM- 20090618
LR  - 20111117
IS  - 1945-7170 (Electronic)
IS  - 0013-7227 (Linking)
VI  - 150
IP  - 6
DP  - 2009 Jun
TI  - Biological and anatomical evidence for kisspeptin regulation of the 
      hypothalamic-pituitary-gonadal axis of estrous horse mares.
PG  - 2813-21
LID - 10.1210/en.2008-1698 [doi]
AB  - The purpose of the present study was to evaluate the effects of kisspeptin (KiSS) 
      on LH and FSH secretion in the seasonally estrous mare and to examine the 
      distribution and connectivity of GnRH and KiSS neurons in the equine preoptic 
      area (POA) and hypothalamus. The diestrous mare has a threshold serum 
      gonadotropin response to iv rodent KiSS decapeptide (rKP-10) administration 
      between 1.0 and 500 microg. Administration of 500 microg and 1.0 mg rKP-10 
      elicited peak, mean, and area under the curve LH and FSH responses 
      indistinguishable to that of 25 microg GnRH iv, although a single iv injection of 
      1.0 mg rKP-10 was insufficient to induce ovulation in the estrous mare. GnRH and 
      KiSS-immunoreactive (ir) cells were identified in the POA and hypothalamus of the 
      diestrous mare. In addition, KiSS-ir fibers were identified in close association 
      with 33.7% of GnRH-ir soma, suggesting a direct action of KiSS on GnRH neurons in 
      the mare. In conclusion, we are the first to reveal a physiological role for KiSS 
      in the diestrous mare with direct anatomic evidence by demonstrating a 
      threshold-like gonadotropin response to KiSS administration and characterizing 
      KiSS and GnRH-ir in the POA and hypothalamus of the diestrous horse mare.
FAU - Magee, Christianne
AU  - Magee C
AD  - Department of Biomedical Sciences, Animal Reproduction and Biotechnology 
      Laboratory, Colorado State University, Fort Collins, Colorado 80523, USA.
FAU - Foradori, Chad D
AU  - Foradori CD
FAU - Bruemmer, Jason E
AU  - Bruemmer JE
FAU - Arreguin-Arevalo, Jesus A
AU  - Arreguin-Arevalo JA
FAU - McCue, Patrick M
AU  - McCue PM
FAU - Handa, Robert J
AU  - Handa RJ
FAU - Squires, Edward L
AU  - Squires EL
FAU - Clay, Colin M
AU  - Clay CM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090219
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (KISS1 protein, human)
RN  - 0 (Kisspeptins)
RN  - 0 (Oligopeptides)
RN  - 0 (Tumor Suppressor Proteins)
RN  - 33515-09-2 (Gonadotropin-Releasing Hormone)
RN  - 9002-67-9 (Luteinizing Hormone)
RN  - 9002-68-0 (Follicle Stimulating Hormone)
SB  - IM
MH  - Animals
MH  - Estrous Cycle/drug effects/*physiology
MH  - Female
MH  - Follicle Stimulating Hormone/blood
MH  - Gonadotropin-Releasing Hormone/metabolism
MH  - Horses/*physiology
MH  - Hypothalamo-Hypophyseal System/drug effects/*physiology
MH  - Hypothalamus/cytology/metabolism
MH  - Injections, Intravenous
MH  - Kisspeptins
MH  - Luteinizing Hormone/blood
MH  - Models, Animal
MH  - Neurons/metabolism
MH  - Oligopeptides/administration & dosage/*physiology
MH  - Ovary/drug effects/*physiology
MH  - Ovulation/drug effects
MH  - Preoptic Area/cytology/metabolism
MH  - Reproduction/physiology
MH  - Tumor Suppressor Proteins/*metabolism
EDAT- 2009/02/21 09:00
MHDA- 2009/06/19 09:00
CRDT- 2009/02/21 09:00
PHST- 2009/02/21 09:00 [entrez]
PHST- 2009/02/21 09:00 [pubmed]
PHST- 2009/06/19 09:00 [medline]
AID - en.2008-1698 [pii]
AID - 10.1210/en.2008-1698 [doi]
PST - ppublish
SO  - Endocrinology. 2009 Jun;150(6):2813-21. doi: 10.1210/en.2008-1698. Epub 2009 Feb 
      19.