PMID- 19229386
OWN - NLM
STAT- MEDLINE
DCOM- 20090504
LR  - 20190608
IS  - 1678-9946 (Electronic)
IS  - 0036-4665 (Linking)
VI  - 51
IP  - 1
DP  - 2009 Jan-Feb
TI  - Classic and molecular study of Giardia duodenalis in children from a daycare 
      center in the region of Presidente Prudente, Sao Paulo, Brazil.
PG  - 19-24
LID - S0036-46652009000100004 [pii]
AB  - Epidemiological studies on giardiasis by using molecular techniques such as RAPD 
      (Randomly Amplified Polymorphic DNA) may give information on factors related to 
      the transmission of Giardia duodenalis. The aim of this work was to assess the 
      epidemiology of G. duodenalis in 101 children attended at a daycare center in 
      Presidente Bernardes, SP, Brazil. After parasitological examinations in feces 
      samples, 15 children presented cysts of G. duodenalis. Their respective parents, 
      brothers and pets, besides the daycare center workers, also had their feces 
      submitted to parasitological analysis. Seven mothers and nine brothers also 
      presented G. duodenalis cysts, while fathers, daycare workers and pets (dogs) did 
      not presented the parasite. Besides the 15 cases with G. duodenalis, other 23 
      children presented other enteroparasites (Entamoeba coli, Endolimax nana, 
      Enterobius vermicularis, Ascaris lumbricoides and Trichuris trichiura). Samples 
      of G. duodenalis cysts from children and their relatives were submitted to 
      molecular typing by RAPD after genomic DNA extraction and amplification of a 
      fragment of the 18S rDNA region by PCR. After examining 31 isolates of G. 
      duodenalis (children and their respective mothers and brothers), it was concluded 
      that the parasite transmission occurred in children, probably during daily 
      cohabitation at the daycare center, but not at home among their relatives or 
      pets.
FAU - Tashima, Nair Toshiko
AU  - Tashima NT
AD  - Departamento de Parasitologia, Universidade do Oeste Paulista, Presidente 
      Prudente, SP, Brasil. nairtashima@unoeste.br
FAU - Simoes, Maria Jacira Silva
AU  - Simoes MJ
FAU - Leite, Clarice Queico Fujimura
AU  - Leite CQ
FAU - Fluminhan, Antonio
AU  - Fluminhan A
FAU - Nogueira, Marco Antonio
AU  - Nogueira MA
FAU - Malaspina, Ana Carolina
AU  - Malaspina AC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Brazil
TA  - Rev Inst Med Trop Sao Paulo
JT  - Revista do Instituto de Medicina Tropical de Sao Paulo
JID - 7507484
RN  - 0 (DNA, Protozoan)
SB  - IM
MH  - Animals
MH  - Brazil/epidemiology
MH  - Child
MH  - Child Day Care Centers/*statistics & numerical data
MH  - Child, Preschool
MH  - DNA, Protozoan/analysis
MH  - Dogs
MH  - Feces/*parasitology
MH  - Female
MH  - Giardia/classification/*isolation & purification
MH  - Giardiasis/diagnosis/*epidemiology/transmission
MH  - Humans
MH  - Infant
MH  - Infant, Newborn
MH  - Male
MH  - Parasite Egg Count
MH  - Polymerase Chain Reaction
MH  - Prevalence
MH  - Random Amplified Polymorphic DNA Technique
MH  - Risk Factors
EDAT- 2009/02/21 09:00
MHDA- 2009/05/05 09:00
CRDT- 2009/02/21 09:00
PHST- 2008/03/19 00:00 [received]
PHST- 2008/12/23 00:00 [accepted]
PHST- 2009/02/21 09:00 [entrez]
PHST- 2009/02/21 09:00 [pubmed]
PHST- 2009/05/05 09:00 [medline]
AID - S0036-46652009000100004 [pii]
AID - 10.1590/s0036-46652009000100004 [doi]
PST - ppublish
SO  - Rev Inst Med Trop Sao Paulo. 2009 Jan-Feb;51(1):19-24. doi: 
      10.1590/s0036-46652009000100004.