PMID- 19230650
OWN - NLM
STAT- MEDLINE
DCOM- 20090526
LR  - 20191210
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 24
IP  - 8
DP  - 2009 Apr 15
TI  - Detecting and differentiating microbes by dendritic cells for the development of 
      cell-based biosensors.
PG  - 2598-603
LID - 10.1016/j.bios.2009.01.017 [doi]
AB  - Dendritic cells (DCs) are a specialized family of antigen presenting cells. They 
      play critical roles in sensing and processing microbial information through a 
      series of pattern recognition receptors (PPRs), including the well-characterized 
      toll-like receptors (TLRs). In this study, we demonstrated the utilization of a 
      DC cell line, DC2.4, as a cell source for the detection and differentiation of 
      microbes towards the development of cell-based biosensors. As a proof of 
      principle, the gram-negative bacteria Escherichia coli K12 strain D21 and its 
      lipopolysaccharide (LPS) mutants were used as model targets. The stimulation of 
      DCs by bacterial strains was monitored by the production of nitric oxide (NO), 
      and the colorimetric Greiss assay was used to quantify the level of NO produced. 
      Our results demonstrated that DCs could detect and differentiate microbes with 
      subtle differences in the composition of specific cell surface components, i.e. 
      LPS, within minutes. Though the current colorimetric-based NO assay limited the 
      detection sensitivity, we showed that DCs were able to detect as low as 2-3 
      bacteria per cell. Furthermore, compared to macrophages, DCs were superior in 
      discriminating LPS mutants. Our study demonstrates that DCs possess great 
      potential as cell sources for the development of novel cell-based biosensors for 
      detecting microbes with high selectivity and sensitivity and rapid 
      responsiveness. In addition, when DCs are coupled with other biosensor platforms, 
      higher sensitivity can be expected.
FAU - Liu, Shili
AU  - Liu S
AD  - Department of Chemical Engineering, University of Washington, Box 351750, 
      Seattle, WA 98195, United States.
FAU - Tran, Kenny K
AU  - Tran KK
FAU - Pan, Steven
AU  - Pan S
FAU - Shen, Hong
AU  - Shen H
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20090123
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
SB  - IM
MH  - Biological Assay/*instrumentation
MH  - Biosensing Techniques/*instrumentation
MH  - Cell Line
MH  - Colony Count, Microbial/*instrumentation
MH  - Colorimetry/*instrumentation
MH  - Dendritic Cells/*microbiology
MH  - Equipment Design
MH  - Equipment Failure Analysis
MH  - Escherichia coli K12/*isolation & purification/physiology
MH  - Humans
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
EDAT- 2009/02/24 09:00
MHDA- 2009/05/27 09:00
CRDT- 2009/02/24 09:00
PHST- 2008/09/19 00:00 [received]
PHST- 2009/01/08 00:00 [revised]
PHST- 2009/01/09 00:00 [accepted]
PHST- 2009/02/24 09:00 [entrez]
PHST- 2009/02/24 09:00 [pubmed]
PHST- 2009/05/27 09:00 [medline]
AID - S0956-5663(09)00039-6 [pii]
AID - 10.1016/j.bios.2009.01.017 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2009 Apr 15;24(8):2598-603. doi: 10.1016/j.bios.2009.01.017. 
      Epub 2009 Jan 23.