PMID- 19230749
OWN - NLM
STAT- MEDLINE
DCOM- 20090518
LR  - 20090316
IS  - 1386-1425 (Print)
IS  - 1386-1425 (Linking)
VI  - 72
IP  - 5
DP  - 2009 Jun
TI  - Study of proteinous and micellar microenvironment using donor acceptor charge 
      transfer fluorosensor N,N-dimethylaminonaphthyl-(acrylo)-nitrile.
PG  - 1103-11
LID - 10.1016/j.saa.2009.01.011 [doi]
AB  - Interaction of charge transfer fluorophore 
      N,N-dimethylaminonaphthyl-(acrylo)-nitrile (DMANAN) with globular proteins Human 
      Serum Albumin (HSA) and Bovine Serum Albumin (BSA) brings forth a marked change 
      in the position and intensity of band maxima both in case of absorption and 
      fluorescence spectra. Spectroscopic approach has been elaborately implemented to 
      explore the binding phenomena of the probe with HSA and BSA and it is found that 
      the extent of binding of the probe to both serum albumins is similar in nature. 
      Steady state fluorescence anisotropy values, fluorescence quenching study using 
      acrylamide quencher and Red Edge Excitation Shift (REES) help in drawing reliable 
      conclusions regarding the location of the probe molecule within the hydrophobic 
      cavity of the proteins. An increase in fluorescence lifetime of the probe 
      molecule solubilized in both the proteinous media also indicate that the probe is 
      located at the motionally restricted environment inside the hydrophobic cavity of 
      proteins and hence non-radiative channels are less operative than in the bulk 
      water. Similarly, the variation of position and intensity of the emission maxima 
      of DMANAN solubilized in micellar medium of Sodium Dodecyl Sulphate (SDS) also 
      predicts well the critical micellar concentration (CMC) and polarity of micellar 
      microenvironment.
FAU - Singh, Rupashree Balia
AU  - Singh RB
AD  - University of Calcutta, Department of Chemistry, 92 A.P.C. Road, Kolkata 700009, 
      India.
FAU - Mahanta, Subrata
AU  - Mahanta S
FAU - Guchhait, Nikhil
AU  - Guchhait N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090124
PL  - England
TA  - Spectrochim Acta A Mol Biomol Spectrosc
JT  - Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
JID - 9602533
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Micelles)
RN  - 0 (Naphthalenes)
RN  - 0 (Nitriles)
RN  - 0 (Serum Albumin)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - *Electron Transport
MH  - Fluorescent Dyes/*chemistry
MH  - Humans
MH  - *Micelles
MH  - Molecular Structure
MH  - Naphthalenes/chemistry
MH  - Nitriles/*chemistry
MH  - Serum Albumin/chemistry
EDAT- 2009/02/24 09:00
MHDA- 2009/05/19 09:00
CRDT- 2009/02/24 09:00
PHST- 2008/06/18 00:00 [received]
PHST- 2009/01/08 00:00 [accepted]
PHST- 2009/02/24 09:00 [entrez]
PHST- 2009/02/24 09:00 [pubmed]
PHST- 2009/05/19 09:00 [medline]
AID - S1386-1425(09)00019-5 [pii]
AID - 10.1016/j.saa.2009.01.011 [doi]
PST - ppublish
SO  - Spectrochim Acta A Mol Biomol Spectrosc. 2009 Jun;72(5):1103-11. doi: 
      10.1016/j.saa.2009.01.011. Epub 2009 Jan 24.