PMID- 19232436
OWN - NLM
STAT- MEDLINE
DCOM- 20100125
LR  - 20220408
IS  - 1872-9142 (Electronic)
IS  - 0161-5890 (Linking)
VI  - 47
IP  - 1
DP  - 2009 Nov
TI  - Proinflammatory and immunoregulatory mechanisms in periapical lesions.
PG  - 101-13
LID - 10.1016/j.molimm.2009.01.011 [doi]
AB  - Proinflammatory and immunoregulatory cytokines are important for the pathogenesis 
      of periapical lesions. However, little is known about how their functions are 
      balanced and controlled at different phases of lesion development. The aim of 
      this study was to examine the relationship between the production of Th1, Th2, 
      Th17 and T regulatory cell (T reg) cytokines by human periapical lesion 
      mononuclear cells (PL-MNC) in culture and their correlation with cellular 
      composition and clinical presentation of the lesions. We show that symptomatic 
      lesions are characterized by the infiltration of neutrophils, high production of 
      IL-17, positive correlation between IL-17 and IFN-gamma, but not between IL-17 
      and IL-23 production. Most IL-17(+) cells coexpressed IFN-gamma. Asymptomatic 
      lesions were phenotypically heterogeneous. The lesions with the predominance of T 
      cells over B cells/plasma cells expressed higher levels of IFN-gamma which 
      correlated with higher production of IL-12 and the frequency of macrophages. In 
      contrast, in most B-type lesions higher levels of IL-5 and TGF-beta were 
      observed, as well as positive correlation between the production of TGF-beta and 
      IL-10. The addition of Th cytokines in PL-MNC cultures confirmed that Th1, Th2 
      and Th17 cytokines are mutually antagonistic, except that IL-17, unexpectedly, 
      augmented the production of IFN-gamma. IL-10 and TGF-beta inhibited the 
      production of both Th1 and Th17 cytokines. Dendritic cells (DCs) from periapical 
      lesions, composed of immature (CD83(-)), and mature (CD83(+)) myeloid type DCs 
      and plasmacytoid (BDCA2(+)) DCs produced higher levels of IL-12 and IL-23 but 
      lower levels of IL-10 and TNF-alpha than monocyte (Mo) -derived DCs. IL-23 
      stimulated the production of IL-17 by PL-MNC, whereas the secretion of IFN-gamma 
      was enhanced by both IL-12 and IL-23. Cumulatively, these results suggest that: 
      (1) Th1 immune response is most probably important for all stages of periapical 
      lesion development; (2) Th2 and immunoregulatory cytokines are more significant 
      for advanced types of lesions with the predominance of B cells/plasma cells; (3) 
      Th17 immune response seems to play a dominant role in exacerbating inflammation.
FAU - Colic, Miodrag
AU  - Colic M
AD  - Institute for Medical Research, Military Medical Academy, Crnotravska 17, 11002 
      Belgrade, Serbia. vmaimi@eunet.rs
FAU - Gazivoda, Dragan
AU  - Gazivoda D
FAU - Vucevic, Dragana
AU  - Vucevic D
FAU - Vasilijic, Sasa
AU  - Vasilijic S
FAU - Rudolf, Rebeka
AU  - Rudolf R
FAU - Lukic, Aleksandra
AU  - Lukic A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090215
PL  - England
TA  - Mol Immunol
JT  - Molecular immunology
JID - 7905289
RN  - 0 (Cytokines)
RN  - 0 (Interleukins)
SB  - IM
MH  - B-Lymphocytes
MH  - Cells, Cultured
MH  - Cytokines/biosynthesis
MH  - Humans
MH  - Inflammation/immunology
MH  - Interleukins/biosynthesis
MH  - Neutrophil Infiltration
MH  - Periapical Diseases/*immunology/*pathology
MH  - Plasma Cells
MH  - T-Lymphocytes, Helper-Inducer/immunology
MH  - T-Lymphocytes, Regulatory/immunology
EDAT- 2009/02/24 09:00
MHDA- 2010/01/26 06:00
CRDT- 2009/02/24 09:00
PHST- 2008/10/09 00:00 [received]
PHST- 2008/12/24 00:00 [revised]
PHST- 2009/01/08 00:00 [accepted]
PHST- 2009/02/24 09:00 [entrez]
PHST- 2009/02/24 09:00 [pubmed]
PHST- 2010/01/26 06:00 [medline]
AID - S0161-5890(09)00034-0 [pii]
AID - 10.1016/j.molimm.2009.01.011 [doi]
PST - ppublish
SO  - Mol Immunol. 2009 Nov;47(1):101-13. doi: 10.1016/j.molimm.2009.01.011. Epub 2009 
      Feb 15.